Primary antibodies were against p19 (NB200‐106, Novus Biologicals, CO, USA), p21 (#2947, Cell Signaling Technology, Beverly, MA, USA), p53 (#2424, Cell Signaling Technology, USA), p16 (ab211542, Abcam, Cambridge, MA, USA), renin (#5250, Cell Signaling Technology, USA), Ang II (NBP1‐31127, Novus Biologicals, USA), ANP (#AB5490, Millipore, MA, USA; sc‐515701, Santa Cruz Biotechnology Inc., Dallas, TX, USA, USA), BNP (#DF6902, Affinity Biosciences, OH, USA; ab19645, Abcam, USA), GATA4 (#19530, Proteintech, IL, USA; sc‐25310, Santa Cruz Biotechnology Inc., USA), LC3B (#NB600‐1384, Novus Biologicals, USA), p62 (#39749, Cell Signaling Technology, USA), Bmi‐1 (#5856, Cell Signaling Technology, USA; 66161‐1‐Ig, Proteintech, USA), RING1B (#5694, Cell Signaling Technology), NF‐κB‐p65 (sc‐8008, Santa Cruz Biotechnology Inc., USA; #8242, Cell Signaling Technology, USA), p‐p65 (Ser536) (ab76302, Abcam, USA), IκB‐α (AF1282, Beyotime Biotechnology, Shanghai, China), p‐IκB‐α (Ser32) (sc‐8404, Santa Cruz Biotechnology Inc., USA), p‐Chk2 (Thr68) (PA5‐104715, Invitrogen Inc. CA, USA), SOD‐2 (NB100‐1992, Novus Biologicals, USA), HSC70 (10654‐1‐AP, Proteintech, USA), p‐ULK1 (Ser757) (#14202, Cell Signaling Technology, USA) and ULK1 (sc‐390904, Santa Cruz Biotechnology, USA). β‐actin (AP0060, Bioworld Technology Inc., MN, USA) was loading control for total protein.
P p65 ser536
Manufactured by Abcam
Sourced in United States, China
P-p65 (Ser536) is a phospho-specific antibody that recognizes the p65 subunit of the NF-κB complex when phosphorylated at serine 536. It is a research-use only product.
Automatically generated - may contain errors
Lab products found in correlation
P ulk1 ser757, by Cell Signaling Technology (1 mentions)
Gata4, by Proteintech (1 mentions)
Nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Hsc70, by Proteintech (1 mentions)
Ring1b, by Cell Signaling Technology (1 mentions)
Ang 2, by Novus Biologicals (1 mentions)
β actin, by Bioworld Technology (1 mentions)
Mmp13, by Proteintech (1 mentions)
Aggrecan, by Wanlei (1 mentions)
Prx 1, by Proteintech (1 mentions)
Sirt1, by Proteintech (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
β actin, by Proteintech (1 mentions)
Bca assay, by Beyotime (1 mentions)
Tnf α, by Proteintech (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Hrp conjugated secondary antibody, by Beyotime (1 mentions)
Il 1β, by Wanlei (1 mentions)
2 protocols using p p65 ser536
1
Comprehensive Protein Analysis in Cardiac Cells
2
Western Blot Analysis of NP Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Intervertebral disc tissue or NP cells were lysed by using RIPA lysis buffer (Beyotime, China) with 1% PI on ice. The protein concentration was determined by using a bicinchoninic acid (BCA) assay (Beyotime, China). 30 μg proteins of each sample were electrophoresed by performing sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) on 10% gels. The separated proteins were transferred onto a PVDF membrane and blocked with 5% bovine serum albumin (BSA, gibco, USA). Then the membranes were incubated overnight at 4 °C with primary antibodies against aggrecan (1:1000 dilution; Wanleibio, China), COL2 (1:1000 dilution; Wanleibio, China), MMP13 (1:1000 dilution; Proteintech, USA), TNF-α (1:1000 dilution; Proteintech, USA), IL-1β (1:1000 dilution; Wanleibio, China), p65 (1:1000 dilution; Abmart, China), p-p65 (Ser536) (1:1000 dilution; Abcam, UK), acetyl-p65 (Lys310) (1:1000 dilution; Abcam, UK), Sirt1 (1:1000 dilution; Proteintech, USA), Prx-1(1:1000 dilution; Proteintech, USA) and β-actin (1:10000 dilution; Proteintech, USA). Finally, the membranes were washed and incubated with HRP-conjugated secondary antibody (1:5000 dilution; Beyotime, China) at room temperature for 2 h. Immunoreactive bands were visualized with ECL chemiluminescence (ChemiDoc XRS+, Bio-Rad, USA) and analyzed by Image lab.
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