Analytical reagent grade chemicals, including ferric chloride anhydrous, sulphuric acid, nitric acid, and hydrochloric acid, were procured from Loba Chemie Private Limited, Mumbai, India. Gum acacia powder, Meuller Hinton’s Agar, potato dextrose agar, Dulbecco’s Modified Eagles Medium, L-glutamine, and streptomycin (sulfate salt) were purchased from Sigma Aldrich, St. Louis, MO, USA. Ciprofloxacin, nutrient agar, nutrient broth, and streptomycin were procured from Hi-Media Private Limited, Mumbai, India. Gram-positive and Gram-negative food pathogenic microbial strains, i.e., Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 443), were obtained from the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. Human colon adenocarcinoma cells (Caco-2 cells) were collected from National Centre for Cell Science, Pune, Maharashtra, India. Plastic dishes, plates, and transwell were obtained from Corning (Corning, NY, USA). Cellular grade, triple distilled water and aqua-regia washed glassware (class “A” certified) were used during research work.
Nitric acid
Manufactured by Loba Chemie
Sourced in India
Nitric acid is a clear, colorless to yellow, fuming, and highly corrosive liquid chemical. It is an inorganic compound with the chemical formula HNO3. Nitric acid is commonly used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Loba Chemie (4 mentions)
Escherichia coli mtcc 443, by Microbial Type Culture Collection (3 mentions)
Staphylococcus aureus mtcc 3160, by Microbial Type Culture Collection (3 mentions)
Ethanol, by Loba Chemie (2 mentions)
Phosphate buffer, by Loba Chemie (2 mentions)
L ascorbic acid, by Merck Group (2 mentions)
Phosphate buffer saline (pbs), by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Loba Chemie (2 mentions)
Citric acid, by Loba Chemie (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Potato dextrose agar (pda), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Sulphuric acid, by Loba Chemie (1 mentions)
Caco 2 cells, by Corning (1 mentions)
Ferric chloride anhydrous, by Loba Chemie (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
5 protocols using nitric acid
1
Antimicrobial and Anticancer Evaluation
2
Standardized Analytical Reagents Procurement
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HPLC grade standards include caffeic acid, syringic acid, p-coumaric acid, rutin, L-ascorbic acid, BSA (bovine serum albumin), DPPH (2-2-diphenyl-1-picrylhydrazyl), and phosphate buffer saline (PBS) were procured from Sigma Aldrich Co. St. Louis, USA. Other 'Analytical Reagent grade chemicals including sodium hydroxide, ethanol, citric acid, hydrochloric acid, nitric acid, phosphate buffer, and diclofenac sodium were procured from Loba Chemie Pvt Ltd. Navi Mumbai, India. For microbiological experiments, standard antibiotics (ciprofloxacin), Mueller Hinton Agar (MHA), nutrient agar, and nutrient broth were purchased from Hi-Media Pvt. Ltd., Mumbai, India. Pathogenic microbial strains Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 443), were procured from the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. For the accomplishment of the research, deionized water and aqua-regia washed class 'A' glassware was used.
3
Comprehensive Analytical Methods for Diverse Samples
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Analytical standard chemicals such as carbon tetrachloride (99.8%, PubChem), Ethanol (96%, Merck), Potassium hydrogen phthalate (>99%, PubChem), potassium hydroxide (85%, Qualikems), sodium hydroxide (≥98%, Blulux Laboratories Ltd), hydrochloric acid (37%, Loba Chemie), phenolphthalein (PubChem), potassium iodide (99%, Alpha Chemika), Nitric acid (69%, Lobachemie), Silver nitrate (99.99%, Alpha Chemika), Acetone (99.99%, Fisher Scientific International Company), Potassium chromate, (99.99%, Fisher Scientific International Company) Light petroleum, Sulphuric acid (98%, Ankleshwar Gujarat, India), Sodium hydrogen carbonate (99%, TATA Chemicals), n-Hexane (99.0%, LobaChemie), methyl orange (Abronchemicals), Chloroform (99.8%, LobaChemie), analytical reagent quality, Glacial acetic acid (99.5%, Blulux laboratories), and Starch solution were used in this study. In addition, a standard food-grade edible vegetable cooking oil, WCOs, Endod berries powder, Fresh ginger, and double distilled water were also used for the study.
4
Synthesis of Conducting Polypyrrole Composites
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Pyrrole monomer and sodium dodecyl sulphate (SDS) were purchased from Sigma Aldrich. Pyrrole was distilled to separate out the polymerized components and a colorless liquid obtained was stored at 0 °C for further use. Ferric chloride (FeCl3) purchased from Merck limited, Mumbai and Nitric acid purchased from Loba Chemie, Mumbai, India were used as received. All these solvents were analytical grade. Carbon cloth (thickness - 0.2 mm) was purchased from Hitsan Incorporation, Chandigarh India.
5
Antioxidant and Antimicrobial Evaluation of Natural Extracts
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L-ascorbic acid, bovine serum albumin, 2-2-diphenyl-1-picrylhydrazyl, gum arabic, 2-thiobarbituric acid, phosphate buffer saline (PBS), and cyclohexanone were purchased from Sigma Aldrich Co. St. Louis, MO, USA. Sodium hydroxide, ethanol, citric acid, hydrochloric acid, nitric acid, Tween 80, phosphate buffer, and ammonium sulfate were purchased from Loba Chemie Pvt Ltd. Navi Mumbai, India. Standard antibiotics (ciprofloxacin), Muller Hinton Agar, nutrient agar, and nutrient broth were procured from Hi-Media Private Limited, Mumbai, India. Gram-positive and Gram-negative bacterial strains, i.e., Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 443), were obtained from the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. Analytical grade chemicals and acid-washed glassware were used throughout the experiments.
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