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Jem 2100f electron microscope

Manufactured by Shimadzu

The JEM-2100F is a high-performance field emission electron microscope manufactured by Shimadzu. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of materials and applications. The JEM-2100F features a field emission electron gun, advanced optics, and a high-resolution detection system to enable detailed examination of samples at the nanoscale level.

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2 protocols using jem 2100f electron microscope

1

Characterization of Nanoparticle Samples

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TEM
was performed using a JEM-2100F electron microscope at a working voltage
of 200 kV. A UV-3101PC Shimadzu spectrometer was used to measure the
UV–vis spectra. A Zetasizer Nanoseries instrument (Nano ZS90)
was used to measure the zeta potential and size distribution. An FV
1000 camera (Olympus) was used to collect CLSM images. An ultrasonic
transducer (Chattanooga Co., USA) was used in all experiments. A Bruker
EMX electron paramagnetic resonance system was used for the ESR.
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2

Characterization of PpIX/3-MA@Lip Nanostructure

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The morphology and structure of PpIX/3-MA@Lip were observed by transmission electron microscopy (TEM) scanning, which was conducted on a JEM-2100F electron microscope operated at 5 kV. UV–vis spectra were performed to evaluate the existence of PpIX on a UV-3101PC Shimadzu spectroscope. High-performance liquid chromatography (HPLC) was employed to determine the encapsulation efficiency of PpIX and 3-MA in the nanoliposome by comparing the area under the curve. The hydrodynamic particle size was measured by Dynamic Light Scattering (DLS) and Zeta potential was tested on a Zetasizer Nanoseries instrument (Nano ZS90).
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