Mabs against cd4 and cd8

Manufactured by Thermo Fisher Scientific

The MAbs against CD4 and CD8 are monoclonal antibodies that target the CD4 and CD8 receptors, respectively. These antibodies are used for the identification and quantification of T-cell subsets in flow cytometry applications.

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Lab products found in correlation

Flojo, by BD (2 mentions)

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CD4 and CD8 (6 citations)

2 protocols using mabs against cd4 and cd8

T Cell Development and Characterization

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Double positive thymocytes were purified by fluorescence activated cell sorting (FACS) using mAbs against CD4 and CD8 (eBioscience). Peripheral naïve CD4⁺ T cells were purified by FACS using mAbs against CD4, CD45RB and CD25. DN3 and DN4 thymocytes were stained with mAbs against Mac1, Ter119, Gr1, B220, panNK, CD11b, CD11c, CD4, CD8 and γδ TCR do determine lineage positive populations. For flow cytometric analysis, mAbs against the following molecules were used: CD4 (RM4-5), CD44 (IM7), CD5 (53-7.3), CD8 (53-6.7), CD25 (PC61), CD69 (H1.2F3), B220 (RA3-6B2), TCRβ (H57), Foxp3 (FJK-16s). Flow cytometric analysis was performed using a LSRII (Becton Dickinson) and data analysis using FloJo.

Bettini M.L., Chou P.C., Guy C., Lee T., Vignali K.M, & Vignali D.A. (2017). CD3 ITAM diversity is required for optimal T cell receptor signaling and thymocyte development. Journal of immunology (Baltimore, Md. : 1950), 199(5), 1555-1560.

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Flow Cytometric Analysis of T Cell Subsets

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Double positive thymocytes were purified by FACS using mAbs against CD4 and CD8 (eBioscience). Peripheral naïve CD4⁺ T cells were purified by FACS using mAbs against CD4, CD45RB and CD25. DN3 and DN4 thymocytes were stained with mAbs against Mac1, Ter119, Gr1, B220, panNK, CD11b, CD11c, CD4, CD8 and γδ TCR do determine lineage positive populations. For flow cytometric analysis, mAbs against the following molecules were used: CD4 (RM4-5), CD44 (IM7), CD5 (53-7.3), CD8 (53-6.7), CD25 (PC61), CD69 (H1.2F3), B220 (RA3-6B2), TCRβ (H57), Foxp3 (FJK-16s). Flow cytometric analysis was performed using a LSRII (Becton Dickinson) and data analysis using FloJo.

Bettini M.L., Guy C., Dash P., Vignali K.M., Hamm D.E., Dobbins J., Gagnon E., Thomas P.G., Wucherpfennig K.W, & Vignali D.A. (2014). Membrane association of the CD3ε signaling domain is required for optimal T cell development and function. Journal of immunology (Baltimore, Md. : 1950), 193(1), 258-267.

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