Nanowizard 3 system

Manufactured by Bruker

Sourced in Germany

The NanoWizard III system is an atomic force microscope (AFM) designed for high-resolution imaging and nanoscale characterization. It features advanced scanning capabilities and a modular design to accommodate various sample types and measurements.

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Lab products found in correlation

Axio observer d1, by Zeiss (1 mentions) Multimode 8 system, by Bruker (1 mentions) Mpms xl, by Quantum Design (1 mentions) Invenio r spectrometer, by Bruker (1 mentions)

Close spelling variants of this product

JPK Nanowizard III system NanoWizard 3 AFM system NanoWizard 3

7 protocols using nanowizard 3 system

Nanomechanical Mapping of Aortic Tissue

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AFM nanoindentation experiments were performed with a NanoWizard III system (JPK, Germany). All measurements were performed on unfixed aorta samples immersed in HBSS solution at room temperature. A spherical colloidal probe with a nominal diameter of 4.5 µm was attached to the cantilever (NovaScan, USA), which had a nominal spring constant of 0.02 N/m.
For each aorta sample, spatial maps of indentation curves were recorded at many random positions of the sample. Typically, each region-of-interest (ROI) consisted of 6 × 6 curves that were recorded on a 20 µm × 20 µm grid. The corresponding step size of approx. 3 µm was chosen to be slightly smaller than the probe diameter. For selected data presented in the Results, larger 18 × 18 maps were stitched from nine smaller ROIs to visualize the spatial variations in the nanomechanical parameters. The indentation curves were recorded for a maximal loading force of 0.5 nN at a velocity of 0.9 µm/s. In the whole experiment, nearly 10,000 indentation curves were collected and analysed.

Targosz-Korecka M., Jaglarz M., Malek-Zietek K.E., Gregorius A., Zakrzewska A., Sitek B., Rajfur Z., Chlopicki S, & Szymonski M. (2017). AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes. Scientific Reports, 7, 15951.

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Atomic Force Microscopy of Candida albicans

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C. albicans cells were grown overnight as described above, concentrated by centrifugation, washed twice in 5 ml acetate buffer (18 mM CH₃COONa, 1 mM CaCl₂, and 1 mM MnCl₂, pH 5.2), and resuspended in 3 ml of the same buffer. The cells were then immobilized on a polydimethylsiloxane (PDMS) stamp, as described before (84 (link), 84 (link)– (link)86 (link)), and immersed in the same acetate buffer. To gain statistical significance, about 10 to 15 cells were analyzed from three independent experiments for each strain and condition. AFM experiments were conducted on a Nanowizard III system from JPK Instruments (Berlin, Germany). We used microlever cantilever (MLCT) probes from Bruker Probes with a measured spring constant (k) ranging from 0.010 to 0.018 N/m. k was measured before each experiment by the thermal noise method (87 (link)). For elasticity measurements, force maps were recorded in force volume mode and analyzed as previously described (84 (link), 85 (link)).

Ene I.V., Walker L.A., Schiavone M., Lee K.K., Martin-Yken H., Dague E., Gow N.A., Munro C.A, & Brown A.J. (2015). Cell Wall Remodeling Enzymes Modulate Fungal Cell Wall Elasticity and Osmotic Stress Resistance. mBio, 6(4), e00986-15.

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Aorta Nanoindentation Experiments

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AFM nanoindentation experiments were performed with a NanoWizard III system (JPK, Germany). All measurements were performed on unfixed aorta samples immersed in Hanks’ balanced salt solution supplemented with 1% fetal bovine serum, 1% penicillin/streptomycin, and 5 mmol/L glucose. A spherical colloidal probe with a nominal diameter of 4.5 μm, attached to a cantilever (NovaScan, USA), with a spring constant of 0.01 N/m, was used in experiments. The indentation curves were recorded for a maximal loading force of 1 nN at a velocity of 1.5 μm/s. For each aorta sample, spatial maps of indentation curves were recorded at many random positions of the sample. Typically, each region of interest comprised 10×10 curves that were recorded on a 20×20 μm grid.

Bar A., Targosz‐Korecka M., Suraj J., Proniewski B., Jasztal A., Marczyk B., Sternak M., Przybyło M., Kurpińska A., Walczak M., Kostogrys R.B., Szymonski M, & Chlopicki S. (2019). Degradation of Glycocalyx and Multiple Manifestations of Endothelial Dysfunction Coincide in the Early Phase of Endothelial Dysfunction Before Atherosclerotic Plaque Development in Apolipoprotein E/Low‐Density Lipoprotein Receptor‐Deficient Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(6), e011171.

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Atomic Force Microscopy Imaging

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AFM experiments were performed using a Nanowizard III system (JPK Instruments, Germany) mounted on an Axio Observer D1 inverted optical microscope (Carl Zeiss, Oberkochen, Germany). The images were acquired in tapping mode in air at room temperature. Single beam silicon cantilevers (TESPA, NanoAndMore USA Corp., Watsonville, CA, USA) with a nominal spring constant of 42 N/m and resonance frequency of 320 kHz were employed. Images with two different scan sizes (2 × 2 μm² and 10 × 10 μm²) were acquired on five different areas on each sample. The scan rate was between 0.5 Hz and 1.0 Hz.

Nanni G., Heredia-Guerrero J.A., Paul U.C., Dante S., Caputo G., Canale C., Athanassiou A., Fragouli D, & Bayer I.S. (2019). Poly(furfuryl alcohol)-Polycaprolactone Blends. Polymers, 11(6), 1069.

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Growth and Characterization of Sn Nanostructures

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Details on the growth and structural characterization of the Sn nanostructures can be found in ref. ^{32 (link)}. The Sn islands were grown using molecular beam epitaxy on Si(111) substrates. The Sn cluster-assembled film was grown on a SiO₂ substrate using a laser-vaporization cluster source⁵¹. The AFM images were recorded using a Nanowizard 3 system (JPK, Germany) and a Multimode 8 system (Bruker, USA) and processed using WSxM^{52 (link)}. The superconducting behaviour of the different Sn samples was probed by SQUID magnetization measurements (LOT-Quantum Design, MPMS-XL). The phonon density of states of all Sn samples was measured using nuclear resonant inelastic X-ray scattering^{53 –55 (link)} at sector 3-ID of the Advanced Photon Source (Argonne National Laboratory, USA). Measurements were carried out at 35 ± 20 K in a grazing incidence geometry. The data were analyzed using the PHOENIX software^{56 (link)}. A more elaborate discussion of the nuclear resonant inelastic X-ray scattering measurements can be found elsewhere^{32 (link)}.

Houben K., Jochum J.K., Couet S., Menéndez E., Picot T., Hu M.Y., Zhao J.Y., Alp E.E., Vantomme A., Temst K, & Van Bael M.J. (2020). The influence of phonon softening on the superconducting critical temperature of Sn nanostructures. Scientific Reports, 10, 5729.

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Multimodal Characterization of Materials

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Atomic force microscopy (AFM) measurements were performed under ambient conditions using a NanoWizard 3 System (NanoWizard 3, JPK Instruments) in tapping mode with a PPP-NCHR AFM probe. Raman spectra were obtained with an InVia Raman microscope with a 532 nm excitation laser, 1800 mm⁻¹ grating and a spatial resolution of 1 mm from Renishaw, Gloucestershire, UK. Fourier-transform infrared spectroscopy (FTIR) was performed at room temperature with a Bruker Invenio R spectrometer. X-ray diffraction (XRD) measurements were carried out on a Panalytical Empyrean 2 diffractometer with a Ge 220 monochromator, CuKα₁ radiation (λ = 0.154056 nm) and a programmable anti-scatter slit.

Roscher S., Hoffmann R., Prescher M., Knittel P, & Ambacher O. (2019). High voltage electrochemical exfoliation of graphite for high-yield graphene production. RSC Advances, 9(50), 29305-29311.

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Elastic Modulus of PEG-SCPs by AFM

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To calculate the adhesion energies of the SCPs, the elastic modulus of the particle is required (see Equation 1). AFM force spectroscopy with a NanoWizard 3 system (JPK instruments AG, Berlin, Germany) was performed to determine the elastic modulus of the microparticles. As AFM probe a glass bead with a diameter of 5.1 µm was glued with an epoxy glue onto a tipless, non-coated cantilever (spring constant 0.32 N/m; CSC12, NanoAndMore GmbH). Several force curves were recorded from different particles and analyzed with an appropriate contact model developed by Glaubitz et al. [27 (link)]. The elastic moduli of PEG-SCPs were 32 ± 5 kPa and showed no systematic variation with regard to grafting type or degree of mannose functionalization.

Schmidt S., Wang H., Pussak D., Mosca S, & Hartmann L. (2015). Probing multivalency in ligand–receptor-mediated adhesion of soft, biomimetic interfaces. Beilstein Journal of Organic Chemistry, 11, 720-729.

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