Materials used in this study were listed as follows: Dulbecco's modified eagle medium (DMEM)-low glucose (HyClone, Logan, UT, USA); DMEM/F12 culture medium (HyClone); B27 supplement without vitamin A (Gibco, New York, NY, USA); recombinant murine epidermal growth factor (EGF) (PeproTech, Rocky Hill, NJ, USA); recombinant murine fibroblast growth factor (FGF)-basic (PeproTech); 0.5% Oil Red O solution (Solarbio, Beijing, China); Masson Stain Kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, Jiangsu, China); cell proliferation assay kit (KeyGen Biotech Co., Ltd., Nanjing, Jiangsu, China); mouse osteogenesis induction and differentiation base media and mouse adipogenesis induction and differentiation base media (Cyagen Biosciences Co., Ltd., Guangzhou, Guangdong, China); anti-mouse Ly-6A/E (stem cell antigen-1, Sca-1) phycoerythrin (PE), anti-mouse CD34 fluorescein isothiocyanate (FITC), anti-mouse CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) FITC, anti-human/mouse CD44 PE (eBioscience, San Diego, CA, USA); anti-nestin antibody, goat anti-mouse immunoglobulin G (IgG)-FITC (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP) (Jackson ImmunoResearch, West Grove, PA, USA); and Alizarin Red S solution (Solarbio, Beijing, China).
Goat anti mouse igg h l horseradish peroxidase hrp
Manufactured by Jackson ImmunoResearch
Sourced in United States
Goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP) is a secondary antibody produced in goats that binds to mouse immunoglobulin (IgG) heavy and light chains. This antibody is conjugated with the enzyme horseradish peroxidase (HRP), which can be used to detect and quantify the presence of mouse IgG in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Oil red o solution, by Solarbio (1 mentions)
Murine recombinant epidermal growth factor, by Thermo Fisher Scientific (1 mentions)
Alizarin red s solution, by Solarbio (1 mentions)
Mouse anti cd31, by Thermo Fisher Scientific (1 mentions)
B 27 supplement without vitamin a, by Thermo Fisher Scientific (1 mentions)
Masson stain kit, by Nanjing Jiancheng (1 mentions)
Benzonase, by Merck Group (1 mentions)
Anti p53 do 1, by Santa Cruz Biotechnology (1 mentions)
Anti prb 4h1, by Cell Signaling Technology (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
2 protocols using goat anti mouse igg h l horseradish peroxidase hrp
1
Isolation and Characterization of Murine Mesenchymal Stem Cells
2
Western Blot Analysis of Protein Samples
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Monolayer cells were washed with 1× phosphate-buffered saline (PBS), harvested in 1.25× Laemmli buffer (78 mM Tris, pH 6.8; 2.5% SDS; 6.25% glycerol; 0.125% bromophenol blue; 2.5% β-mercaptoethanol), and immediately incubated at 99°C for 5 min and then placed on ice. Samples were treated with 100 U/ml Benzonase (Millipore) for 5 min at room temperature, and protein concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific). Either 30 or 50 µg protein (as indicated in the figure legends) per lane was applied for Western blotting with the following antibodies: anti-FLAG M2 (Sigma-Aldrich catalog number F3165), anti-Strep-tag (IBA catalog number 2-1509-001), anti-p53 DO-1 (Santa Cruz Biotechnology catalog number sc-126), anti-pRb 4H1 (Cell Signaling Technology catalog number 9309), anti-HPV16 E7, clone NM2 (gift from Martin Müller, DKFZ), anti-HPV16 E6 (Euromedex catalog number E6-6F4), anti-kynureninase E-5 (Santa Cruz Biotechnology catalog number sc-390360), anti-CPPED1 H-11 (Santa Cruz Biotechnology catalog number sc-514222), anti-l- Plastin B-9 (Santa Cruz Biotechnology catalog number sc-133218), anti-transgelin 6G6 (Santa Cruz Biotechnology catalog number sc-53932), and goat-anti mouse IgG (H+L) horseradish peroxidase (HRP; Jackson ImmunoResearch).
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