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Cd 31 ab24590

Manufactured by Abcam
Sourced in United States

CD-31 (ab24590) is an antibody that recognizes the CD31 protein, also known as PECAM-1. CD31 is a cell adhesion molecule expressed on the surface of various cells, including endothelial cells, platelets, and certain immune cells. This antibody can be used in techniques such as immunohistochemistry and western blotting to detect and study the expression of the CD31 protein.

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2 protocols using cd 31 ab24590

1

Characterization of Tight Junction Proteins

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Antibodies were obtained from the following sources: GLUT-1 (07-1401), Claudin-5 (ABT45) were purchased from Millipore (Temecula, CA, United States); GAPDH (ab8245), CD-31 (ab24590), Dylight-488-labeled goat anti-rabbit IgG (Abacm,ab96899) and Dylight-594-labeled goat anti-mouse IgG (Abacm,ab96873) were purchased from Abcam (San Francisco, CA, United States); AQP-4 (A5971) and GFAP (G6171) from Sigma (St Louis, MO, United States); Occludin from Thermo Fisher (Waltham, MA, United States); ZO-1 (GTX108613) from GenTex (Irvine, CA, United States); and VE-cadherin (ARG20566) from Arigo (Hsinchu, Taiwan, China).
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2

Antibody Reagents for TGF-β Signaling

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PIO and GW9662 (LTD >98%) were purchased from Sigma-Aldrich. Recombinant human TGF-β1 protein (ab50036) was purchased from Abcam. Antibodies against the following proteins were purchased from Abcam: TGF-β1 (ab64715), vimentin (ab92547), α-SMA (ab5694), and CD31(ab24590). The following antibodies were purchased from Cell Signaling Technology: phospho-(P-)smad2 (3101), total-(T-)smad2 (3103s), P-smad3 (8769), T-smad3 (39513s), and GAPDH (2118). The antibodies against PPAR-γ (sc-7196) were purchased from Santa Cruz Biotechnology. All the secondary antibodies used in the research were obtained from LI-COR Biosciences. The BCA protein assay kit obtained from Pierce (Rockford, IL, USA).
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