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3 protocols using geraniol

1

Comprehensive Microbial and Chemical Analysis

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Plate count agar, potato dextrose agar, xylose lysine desoxycholate agar, Baird–Parker agar, Luria–Bertani broth, and other reagents including NaCl, KH2PO4, glucose, and peptone were purchased from Land Bridge Biotechnology Co., Ltd. (Beijing, China). Phenylethyl alcohol, linalool, 2-heptanol, 4-ethylguaiacol, (E)-2-nonenal, α-terpineol, geraniol, ethanol, and acetic acid were purchased from Macklin (Shanghai, China). Heptanoic acid methyl ester was obtained from Sigma-Aldrich (Shanghai, China). The purity of all the above chemicals was higher than 98%. Other chemicals were of analytical grade and obtained from the Chron Chemical Reagent Co., Ltd. (Chengdu, China).
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2

Chemoenzymatic Synthesis of (Z/E)-3''-OH-GHQ

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(Z/E)-3’’-hydroxy-geranylhydroquinone [(Z/E)-3’’-OH-GHQ] was chemically synthesized as described previously (Oshikiri et al., 2020 (link)). Cofactors FMN, NAD(P)+ and NAD(P)H were purchased from Solarbio (Beijing, China). Deuterium oxide and the substrates cinnamyl alcohol, p-coumaryl alcohol, coniferyl alcohol, sinapyl alcohol, and geraniol were purchased from Macklin (Shanghai, China).
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3

Synthesis of Pyraclostrobin Precursors

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Citronella oil was purchased from AFU (Beijing, China) as a mixture without puri cation. Geraniol, citronellol and citronellal were purchased from MACKLIN (China). Pyraclostrobin was purchased from J&K (China). For all reactions, the solvents and chemical reagents, obtained from China National Medicines Co., Ltd, Beijing, China, were of analytical or synthetic grade and were used without puri cation. Column chromatography puri cation was performed using silica gel. NMR spectra were obtained using a Bruker Avance DPX300 spectrometer with tetramethylsilane as the internal standard. Mass spectra were obtained with an Agilent 1100 LC-MSD-Trap mass spectrometer equipped with a standard electrospray ionization (ESI) source.
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