Fear conditioning 2

Manufactured by Ugo Basile

The Fear Conditioning 2.1 is a lab equipment designed for the study of fear-related behaviors in animal models. The system allows for the controlled presentation of conditioned and unconditioned stimuli, enabling the assessment of various parameters associated with fear conditioning.

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Lab products found in correlation

Nidaq 6363, by National Instruments (1 mentions) Ethovision software, by Noldus (1 mentions)

2 protocols using fear conditioning 2

Contextual Fear Conditioning in Mice

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The apparatus and general procedures for contextual fear conditioning (CFC) consisted of a Ugo Basile, ANY-maze controlled Fear Conditioning System (Fear Conditioning 2.1), which entailed a sound-attenuating box, with ventilating fan, a dual (visible/I.R.) light and a USB-camera. Each unit has an individual controller on-board. Mice were tested in a conditioning chamber (17 × 17 × 25(h) cm), containing a stainless-steel shock-grid floor. The detection of freezing is automated and based on video analysis using the Ethovision XT software®. The context consisted in black and white squares decorated walls in the cages.

Ruiz S.A., Tikochinsky E., Rubovitch V., Pick C.G, & Attali B. (2023). Contextual fear response is modulated by M-type K+ channels and is associated with subtle structural changes of the axon initial segment in hippocampal GABAergic neurons. AIMS Neuroscience, 10(1), 33-51.

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Multimodal Monitoring of Post-Stroke Sleep and Behavior

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Sleep recordings: EEG and EMG signals were amplified (× 1,000) using a multichannel differential amplifier (model 3500, AM System) and digitized at 512 Hz (NIDAQ 6363, National Instruments) using a sleep recording software (Sleep Score, View Point). A 48 hours' baseline of spontaneous sleep-wake behaviour were recorded for all animals. Following, SHAM and Opto-STROKE animals were recorded in the infarct's acute phase, or rather during stroke induction and for the following 12 hours (day 0), and semi-chronic phase at days five, ten, fifteen and twenty post-stroke, to follow the progression of sleep behaviours and oscillations.
Behavioural tests: Anxiety-, motor-, cognitive-related outcomes were assessed in sleep recordings-free days. Video recordings of the performances were analysed with Ethovision software (Noldus), allowing the tracking of the mice movements within the whole area and predetermined fractions of the arenas, such centre in the open filed test. For the pain sensitivity testing sessions, videos were recorded via an USB-camera included in the fear conditioning system (Fear Conditioning 2.1, Ugo Basile).

Lenzi I., Borsa M., Czekus C., Rusterholz T., Bassetti C.L., & Herrera C.G. (2021). Optical mini-stroke of thalamic networks impairs sleep stability, topography and cognition.

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