Log In Sign up
The largest database of trusted experimental protocols

Mouse anti h3cit monoclonal antibody

Manufactured by BioCat
Visit Supplier

The Mouse anti-H3Cit monoclonal antibody is a laboratory reagent used for the detection and analysis of histone H3 proteins that have been citrullinated (converted from arginine to citrulline) at specific sites. This antibody is designed to specifically bind to the citrullinated form of histone H3, allowing researchers to study this post-translational modification and its biological implications.

Automatically generated - may contain errors

Lab products found in correlation

In cell analyzer 2000, by GE Healthcare (2 mentions) Hoechst 33342 staining, by Thermo Fisher Scientific (2 mentions) In cell investigator software, by GE Healthcare (2 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions)

2 protocols using mouse anti h3cit monoclonal antibody

1

Quantification of Histone H3 Citrullination

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human neutrophils were isolated as above and diluted to a density of 3.5×105/mL. Cells were incubated with compounds in 384-well micro-titre plates for 45 min at 37 °C, 5% CO2. Cells were then stimulated with 2 µM calcium ionophore (final concentration) for 60 min at 37 °C. The cells were fixed with 1.3% PFA for 45 min at RT, then permeabilised in PBS/2% Triton X-100 for 10 min. Cells were washed with PBST (PBS/0.1% Tween 20) and blocked in PBST/2% BSA for 16 h at 4 °C. H3 citrullination was measured using a mouse anti-H3Cit monoclonal antibody (BioCat, 133483, generated at GSK, 6 µg/mL) and a secondary Alexa Fluor 488 goat anti-mouse IgG (Life Technologies, A11001, 2 µg/mL) with parallel Hoechst 33342 staining (Life Technologies). Plates were imaged on an IN Cell Analyzer 2000 (GE Healthcare) and the FITC intensity in neutrophil nuclei was used as a measure of H3 citrullination as determined using the IN Cell Investigator software (GE Healthcare).
Lewis H.D., Liddle J., Coote J.E., Atkinson S.J., Barker M.D., Bax B.D., Bicker K.L., Bingham R.P., Campbell M., Chen Y.H., Chung C.W., Craggs P.D., Davis R.P., Eberhard D., Joberty G., Lind K.E., Locke K., Maller C., Martinod K., Patten C., Polyakova O., Rise C.E., Rüdiger M., Sheppard R.J., Slade D.J., Thomas P., Thorpe J., Yao G., Drewes G., Wagner D.D., Thompson P.R., Prinjha R.K, & Wilson D.M. (2015). Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation. Nature chemical biology, 11(3), 189-191.
+ Open protocol
+ Expand
2

Quantification of Histone H3 Citrullination

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human neutrophils were isolated as above and diluted to a density of 3.5×105/mL. Cells were incubated with compounds in 384-well micro-titre plates for 45 min at 37 °C, 5% CO2. Cells were then stimulated with 2 µM calcium ionophore (final concentration) for 60 min at 37 °C. The cells were fixed with 1.3% PFA for 45 min at RT, then permeabilised in PBS/2% Triton X-100 for 10 min. Cells were washed with PBST (PBS/0.1% Tween 20) and blocked in PBST/2% BSA for 16 h at 4 °C. H3 citrullination was measured using a mouse anti-H3Cit monoclonal antibody (BioCat, 133483, generated at GSK, 6 µg/mL) and a secondary Alexa Fluor 488 goat anti-mouse IgG (Life Technologies, A11001, 2 µg/mL) with parallel Hoechst 33342 staining (Life Technologies). Plates were imaged on an IN Cell Analyzer 2000 (GE Healthcare) and the FITC intensity in neutrophil nuclei was used as a measure of H3 citrullination as determined using the IN Cell Investigator software (GE Healthcare).
Lewis H.D., Liddle J., Coote J.E., Atkinson S.J., Barker M.D., Bax B.D., Bicker K.L., Bingham R.P., Campbell M., Chen Y.H., Chung C.W., Craggs P.D., Davis R.P., Eberhard D., Joberty G., Lind K.E., Locke K., Maller C., Martinod K., Patten C., Polyakova O., Rise C.E., Rüdiger M., Sheppard R.J., Slade D.J., Thomas P., Thorpe J., Yao G., Drewes G., Wagner D.D., Thompson P.R., Prinjha R.K, & Wilson D.M. (2015). Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation. Nature chemical biology, 11(3), 189-191.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!