C57BL/6J WT mice, B6.129-Prdm1tm1Clme/J (Prdmfl/fl) mice, B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (Cd4cre) mice, and B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) mice were purchased from Jackson Laboratory. Gpr120−/− mice were provided by Bristol-Myers Squibb. Gpr120fl/fl mice were generated by inserting two loxP sites in the Gpr120 allele using CRISPER/Cas9 gene-editing technology in C57BL/6J background in Viewsolid Biotech and crossed to Cd4cre to generate Cd4creGpr120fl/fl mice. IL-10−/− mice were provided by Dr. Cohn at the University of the Texas Medical Branch (UTMB). Prdm1fl/fl mice were crossed to Cd4cre to generate Cd4crePrdmfl/fl mice. All the mice were maintained on a 12 hour-light/dark cycle with the temperature of 20–26°C and 30–70% humidity in the specific pathogen-free animal facility of UTMB. All the experimental mice were sex-matched and age-matched littermates and cohoused after weaning. All the animal experiments were reviewed and approved by the Institutional Animal Care and Use Committee of UTMB.
B6 cg tg cd4 cre 1cwi bfluj cd4cre mice
Manufactured by Jackson ImmunoResearch
Sourced in United Kingdom
The B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (Cd4cre) mice are a transgenic mouse strain that express the Cre recombinase enzyme under the control of the Cd4 promoter. The Cd4 gene is expressed in T cells, and the Cre recombinase can be used to generate conditional gene deletions or modifications in these cells.
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2 protocols using b6 cg tg cd4 cre 1cwi bfluj cd4cre mice
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Genetically Modified Mouse Models
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Genotyping of Bcl6 Knockout Mice
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All animal work was conducted in accordance with the UK Home Office and the local AWERB committee (UK Home Office project license P996A24e1). Specific pathogen–free 6- to 8-wk-old or pregnant female BALB/c and C57BL/6 mice were purchased from Charles River Laboratories. 6- to 10-wk-old B6.129S-Bcl6tm1.1Dent/J (Bcl6fl) mice (Hollister et al., 2013 (link); stock number 023727) were purchased from The Jackson Laboratory and bred with B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (CD4Cre) mice (Lee et al., 2001 (link); stock number 022071; kindly provided by Professor Matthias Merkenschlager, Imperial College London, London, UK) at Central Biomedical Services (Imperial College London, UK) and Charles River Laboratories (Margate, UK). Genotypes were determined using DNA extracted from ear biopsies processed using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich), amplified by PCR, and separated by gel electrophoresis according to the Bcl6fl and Generic Cre protocols provided by The Jackson Laboratory; custom PCR primers were purchased from Sigma-Aldrich.
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