Blood urea nitrogen (BUN) and serum creatinine were measured using specific assay kits (#K002-H1, Arbor Assays, Ann Arbor, MI, USA). TNF-α (#BMS607-3), IL-6 (#KHC-0061), and IFN-γ (#KMC4021, Invitrogen, Carlsbad, CA, USA) were measured using specific ELISA kits following the manufacturer′s instructions.
Khc0061
Manufactured by Thermo Fisher Scientific
Sourced in United States
The KHC0061 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for conducting specific laboratory procedures and analyses. The product's core function is to assist in the processing and handling of samples within a controlled laboratory setting. Further details about the intended use or specific applications of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Bms607 3, by Thermo Fisher Scientific (1 mentions)
Kmc4021, by Thermo Fisher Scientific (1 mentions)
Ab46052, by Abcam (1 mentions)
Ab46087, by Abcam (1 mentions)
Ab46032, by Abcam (1 mentions)
Hcytomag 60k, by Merck Group (1 mentions)
Kac1591, by Thermo Fisher Scientific (1 mentions)
Dtslp0, by R&D Systems (1 mentions)
Bms223hs, by Thermo Fisher Scientific (1 mentions)
Bms224hs, by Thermo Fisher Scientific (1 mentions)
Elx800, by Agilent Technologies (1 mentions)
4 protocols using khc0061
1
Biomarker Measurement Protocol
2
Cytokine Profiling in Cell-Free Sputum Supernatants
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In cell-free sputum supernatants, the following cytokines were measured with ELISA kits according to the manufacturer's instructions: IL-1β (ab46052; Abcam, Cambridge, UK), IL-5 (ab46026; Abcam), IL-6 (KHC0061; Invitrogen, Camarillo, CA, USA), IL-8 (ab46032; Abcam), IL-13 (ab46038; Abcam), IL-17 (KAC1591; Invitrogen), IL-25 (EK0793; Boster Biotech; Pleasanton, CA, USA), IL-33 (SEB980Hu; Cloud-Clone Corp., Katy, TX, USA), TNF-α (ab46087; Abcam), TSLP (DTSLP0; R&D Systems, Minneapolis, MN, USA), GM-CSF (HCYTOMAG-60K; EMD Millipore, Darmstadt, Germany), and IFN-γ (HCYTOMAG-60K; EMD Millipore). Cytokine levels were normalized to protein concentrations in the sputum.
3
Cytokine Quantification by ELISA
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The concentrations of IL-1β, IL-6 and TNF-α were determined using ELISA kits (Invitrogen BMS224HS, KHC0061, BMS223HS; Austria). According to the manufacturer' s instructions, a colored product was formed in proportion to the amount of cytokines present in the sample or standard. The reaction was terminated by Marmara Med J 2020;33(3): 146-152
4
Quantification of IL-6 in Culture Supernatants
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The analysis of IL-6 was performed in the culture supernatants. The culture supernatants were centrifuged. In the supernatants, IL-6 level was assayed using appropriate ELISA kit (KHC0061, Invitrogen, USA). The analyses were performed according to the instructions and read by the absorbance microplate reader (BioTek, EL x 800). The concentration of total protein in the supernatant was detected by the Breadford method.
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