Anti irf3 s396 p

The following antibodies were used in the study: anti-Strep Tag (Sigma, SAB2702216), anti-FLAG (Sigma, F1804), anti-V5 (Invitrogen, MA5-15253), anti-IRF3 (Cell Signaling, 4302S), anti-HDAC2 (Abcam, ab124974), anti-Tubulin (Sigma, T5326), anti-STAT1 (Cell Signaling, 14994), anti-STAT1-P (Cell Signaling, 9167), anti-IRF3-S386-P (Abcam, ab76493), and anti-IRF3-S396-P (Cell Signaling, 4947S). Human IFNβ was from Peprotech (300-02BC). The HDAC2 inhibitor Santacruzamat A was from Selleckchem.com (S7595). The HDAC2 siRNA was purchased from Santa Cruz Biotechnology (sc-29345). Lipofectamine RNAiMAX (Invitrogen) was used for the siRNA transfections, which were performed according to the manufacturer’s instructions. 3×FLAG-NSP5 was expressed from the pCDH-CMV-MCS-EF1-Puro vector. The expression plasmids for 2×Strep tagged NSP5, NSP9, NSP10, and EGFP were purchased from Addgene. FLAG-IRF3 and IFNβ-Luc plasmids were gifts from Katherine A. Fitzgerald (UMass Medical School) and Zhijian Chen (UT Southwestern), respectively. The ISRE-Luc reporter vector and the HA-MeV-V expression plasmid were provided by Takeshi Saito (University of Southern California) and Michaela Gack (Cleveland Clinic Florida Research & Innovation Center), respectively.

The following antibodies were used in the study: anti-Strep Tag (Sigma, SAB2702216), anti-FLAG (Sigma, F1804), anti-V5 (Invitrogen, MA5-15253), anti-IRF3 (Cell Signaling, 4302S), anti-HDAC2 (Abcam, ab124974), anti-Tubulin (Sigma, T5326), anti-STAT1 (Cell Signaling, 14994), anti-STAT1-P (Cell Signaling, 9167), anti-IRF3-S386-P (Abcam, ab76493), and anti-IRF3-S396-P (Cell Signaling, 4947S). Human IFNβ was from Peprotech (300-02BC). The HDAC2 inhibitor Santacruzamat A was from Selleckchem.com (S7595). The HDAC2 siRNA was purchased from Santa Cruz Biotechnology (sc-29345). Lipofectamine RNAiMAX (Invitrogen) was used for the siRNA transfections, which were performed according to the manufacturer’s instructions. 3×FLAG-NSP5 was expressed from the pCDH-CMV-MCS-EF1-Puro vector. The expression plasmids for 2×Strep tagged NSP5, NSP9, NSP10, and EGFP were purchased from Addgene. FLAG-IRF3 and IFNβ-Luc plasmids were gifts from Katherine A. Fitzgerald (UMass Medical School) and Zhijian Chen (UT Southwestern), respectively. The ISRE-Luc reporter vector and the HA-MeV-V expression plasmid were provided by Takeshi Saito (University of Southern California) and Michaela Gack (Cleveland Clinic Florida Research & Innovation Center), respectively.