SACC cells were cultured in 12-well cell culture plates. Upon reaching 70% confluence, cells were washed in cold PBS and fixed in 4% paraformaldehyde for 30 min, permeabilized in 0.25% Triton X-100 in PBS for 15 min, and blocked with 1% bovine serum albumin prepared in PBS for 30 min. Lastly, cells were incubated overnight with mouse anti-Ki-67 (1:100 dilution), and then incubated with FITC or TRITC-conjugated goat anti-mouse IgG (1:500; Zhongshan Goldenbridge Biotechnology) at 37 °C for 1 h. Cells were visualized using the Olympus Fluoview confocal microscope (Tokyo, Japan), and fluorescence images were taken.
Tritc conjugated goat anti mouse igg
Manufactured by Zhongshan Golden Bridge Biotechnology
Sourced in Japan
TRITC-conjugated goat anti-mouse IgG is a secondary antibody that is used to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays and imaging techniques. The antibody is conjugated with the fluorescent dye TRITC (Tetramethylrhodamine), which emits red fluorescence upon excitation, allowing for the detection and localization of mouse IgG in samples.
Automatically generated - may contain errors
Lab products found in correlation
Fitc conjugated and tritc conjugated goat anti rabbit igg, by Zhongshan Golden Bridge Biotechnology (2 mentions)
Tritc conjugated donkey anti human igg, by Zhongshan Golden Bridge Biotechnology (2 mentions)
Mouse monoclonal anti α tubulin fitc antibody, by Merck Group (2 mentions)
Fluoview confocal microscope, by Olympus (1 mentions)
Phospho aurora a thr288 aurora b thr232 aurora c thr198, by Cell Signaling Technology (1 mentions)
Rabusertib, by MedChemExpress (1 mentions)
Anti map1lc3a, by Abcam (1 mentions)
Anti rad51 antibody, by Proteintech (1 mentions)
Alexa fluor 594 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Sheep polyclonal anti bubr1 antibody, by Abcam (1 mentions)
Rabbit anti α tubulin monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti α tubulin fitc antibody, by Merck Group (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Alexa fluor 488 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti γ tubulin antibody, by Merck Group (1 mentions)
Mouse anti β actin polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
4 protocols using tritc conjugated goat anti mouse igg
1
Immunofluorescence Assay for Ki-67 Expression
2
Antibodies and Reagents for Cell Biology
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The CHK1 inhibitor Rabusertib was purchased from MedChemExpress. Sheep polyclonal anti‐BubR1 antibody, rabbit monoclonal anti‐γ‐H2A.X and anti‐MAP1LC3A antibodies were obtained from Abcam. The anti‐α‐tubulin‐FITC antibody and Hoechst 33342 were purchased from Sigma. AlexaFluor 488 goat anti‐rabbit antibody and AlexaFluor 594 goat anti‐rabbit antibody were obtained from Invitrogen. The human anti‐centromere CREST antibody was purchased from Fitzgerald Industries International. Mouse polyclonal anti‐BAX, rabbit polyclonal anti‐γ‐H2A.X antibody and anti‐Rad51 antibody were purchased from Proteintech. Rabbit monoclonal anti‐α‐tubulin antibody and phospho‐Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) were purchased from Cell Signaling Technology. FITC‐conjugated and TRITC‐conjugated goat anti‐rabbit IgG, TRITC‐conjugated goat anti‐mouse IgG and TRITC‐conjugated donkey anti‐human IgG were obtained from Zhongshan Golden Bridge Biotechnology, Co., Ltd. All other chemicals and reagents were from Sigma‐Aldrich Corp.
3
Immunofluorescence Staining of Microtubules
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Rabbit polyclonal anti-Kif4a, mouse monoclonal anti-acetylated tubulin antibodies were from Santa Cruz (Santa Cruz, CA, USA). Human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Davis, CA, USA). Mouse monoclonal anti-α-tubulin-FITC antibodies were from Sigma-Aldrich Corp. (St. Louis, MO, USA). FITC-conjugated and TRITC-conjugated goat anti-rabbit IgG, TRITC-conjugated goat anti-mouse IgG and TRITC- conjugated donkey anti-human IgG were from Zhongshan Golden Bridge Biotechnology, Co., Ltd. (Beijing). All other chemicals and reagents were from Sigma-Aldrich Corp., unless otherwise stated.
4
Immunofluorescence and Western Blot Analyses
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Mouse polyclonal anti-CenpH antibody used for western blot and immunofluorescence was purchased from Abcam (ab88593); mouse monoclonal anti-α-tubulin-FITC antibody and mouse monoclonal anti-γ-tubulin antibody were obtained from Sigma-Aldrich (F2168 and T6557); human polyclonal anti-ACA antibody was purchased from Antibodies Incorporated (15-234); mouse monoclonal anti-cyclin B1 antibody was purchased from Santa Cruz (sc-245); rabbit polyclonal anti-Cdc2 (Cdk1) p34 (Tyr15) antibody was purchased from Santa Cruz (sc-12340-R); mouse polyclonal anti-β-actin antibody was purchased from Santa Cruz (sc-8432); FITC-conjugated goat anti-mouse IgG and TRITC-conjugated goat anti-mouse IgG were purchased from Zhongshan Golden Bridge Biotechnology (ZF-0312 and ZF-0313).
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