For Western blot analysis, anti-cleaved PARP (9542), anti-cleaved Caspase 8 (9496), anti-cleaved Caspase 3 (9664), anti-Bax (2772), and anti-Bak (3814), antibodies were purchased from Cell Signaling Technology; anti-Bax (610982) antibodies were from BD Bioscience; anti-PHB1 (10787-1-AP) and anti-PHB2 (12295-1-AP) antibodies were from ProteinTech; anti-PHB1 (sc-377037), anti-PHB2 (sc-133094), anti-TOM20 (F-10) (sc-17764) monoclonal antibodies and anti-eEF2 (H-118; sc-118), anti-Drp1 (H-300; sc-32898) antibodies were from Santa Cruz Biotechnology; anti-Bak (Ab-2; TC-102) monoclonal antibody was from CALBIOCHEM; anti-ROCK1 (GTX113266) and anti-GAPDH (GTX100118) antibodies were from GeneTex; anti-FLAG antibody (018-22381) was from WAKO Pure Chemical; anti-Myc tag antibody (M192-3) was from MBL; HRP-conjugated anti-mouse IgG (HAF007), anti-rabbit IgG (HAF008) antibodies, and Z-VAD-FMK were purchased from R&D Systems; anti-GST HRP (RPN1236) and Streptavidin-HRP were obtained from GE Healthcare Life Sciences. Y27362 (ROCK inhibitor) was from Cayman Chemical. 6-Biotin-17-NAD+ (biotinyl-NAD+) was obtained from Trevigen. CHX was obtained from Sigma Aldrich. Pseudomonas aeruginosa exotoxin A (PEA) was from LIST Biological Laboratories, Inc.
Anti rabbit igg haf008
Manufactured by R&D Systems
Sourced in United States
Anti-rabbit IgG (HAF008) is a laboratory product that can be used to detect and bind to rabbit immunoglobulin G (IgG) antibodies. It is designed for use in various immunological techniques.
Automatically generated - may contain errors
Lab products found in correlation
Z vad fmk, by R&D Systems (1 mentions)
Anti gapdh gtx100118, by GeneTex (1 mentions)
Anti bak 3814, by Cell Signaling Technology (1 mentions)
Streptavidin hrp, by GE Healthcare (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Ly228820, by Selleck Chemicals (1 mentions)
Anti goat igg haf109, by R&D Systems (1 mentions)
Anti mouse igg haf007, by R&D Systems (1 mentions)
Anisomycin, by Enzo Life Sciences (1 mentions)
Sb202190, by Selleck Chemicals (1 mentions)
Anti gapdh 5g4, by HyTest (1 mentions)
2 protocols using anti rabbit igg haf008
1
Antibody Sources for Western Blot Analysis
2
Reagents and Antibodies for P38 Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
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P38 inhibitors were purchased as follows: LY228820 and SB202190 from Selleck Chemicals (Houston, TX, USA); Doramapimod from Medichem Express (Princeton, NJ, USA) and dissolved in DMSO. Anisomycin was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and also solved in DMSO. Primary antibodies were purchased from the following providers: anti-DKK-1 (AF1096), anti-p38α (AF8691) and anti-p38γ (AF1347) from R&D Systems, Inc. (Minneapolis, MN, USA); anti-HSP27 (#2402), anti-p-HSP27 (#2405), anti-p38 (#2121) and anti-p-38 (#9211) from Cell Signaling Technology, Inc. (Beverly, MA, USA); anti-GAPDH (#5G4) from HyTest Ltd (Intelligate, Turku, Finland). Secondary HRP-conjugated antibodies: anti-goat IgG (HAF109), anti-mouse IgG (HAF007) and anti-rabbit IgG (HAF008) where purchased from R&D Systems, Inc. Control antibody for in vitro experiments: polyclonal goat IgG (AB-108-C) from R&D Systems, Inc. Recombinant bone morphogenetic protein 2 (BMP-2) was purchased from Peprotech (Rocky Hill, NJ, USA) and solved in water containing 0.1% bovine serum albumin (BSA) as the carrier protein.
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