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Stainalive ssea 4 mouse anti human antibody

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StainAlive SSEA-4 mouse anti-human antibody is a laboratory reagent used for the detection and identification of SSEA-4, a cell surface marker expressed in human embryonic stem cells and some cancer cells. It is a monoclonal antibody produced in mice.

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Lab products found in correlation

Alkaline phosphatase live stain kit, by Thermo Fisher Scientific (2 mentions) Bz x700 series microscope, by Keyence (2 mentions)

2 protocols using stainalive ssea 4 mouse anti human antibody

1

Alkaline Phosphatase and SSEA-4 Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alkaline Phosphatase detection was executed using the Alkaline Phosphatase Live Stain kit (Life Technologies, Cat. # A14353) according to manufacturer’s instructions. Cells were visualized using a Keyence BZ-X700 series microscope. SSEA-4 detection was carried out using the StainAlive SSEA-4 mouse anti-human antibody (Reprocell, Cat. # 09–0097). Antibody was diluted 1:200 in mTeSR1 and cells were incubated in antibody containing medium for 30 min in standard culture conditions. The staining medium was removed and the cells washed gently 2 times with mTeSR1. Fresh media was added and the cells were visualized using a Keyence BZ-X700 series microscope.
Kuang Y.L., Munoz A., Nalula G., Santostefano K.E., Sanghez V., Sanchez G., Terada N., Mattis A.N., Iacovino M., Iribarren C., Krauss R.M, & Medina M.W. (2019). Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation. Stem cell research, 37, 101434.
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2

Alkaline Phosphatase and SSEA-4 Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alkaline Phosphatase detection was executed using the Alkaline Phosphatase Live Stain kit (Life Technologies, Cat. # A14353) according to manufacturer’s instructions. Cells were visualized using a Keyence BZ-X700 series microscope. SSEA-4 detection was carried out using the StainAlive SSEA-4 mouse anti-human antibody (Reprocell, Cat. # 09–0097). Antibody was diluted 1:200 in mTeSR1 and cells were incubated in antibody containing medium for 30 min in standard culture conditions. The staining medium was removed and the cells washed gently 2 times with mTeSR1. Fresh media was added and the cells were visualized using a Keyence BZ-X700 series microscope.
Kuang Y.L., Munoz A., Nalula G., Santostefano K.E., Sanghez V., Sanchez G., Terada N., Mattis A.N., Iacovino M., Iribarren C., Krauss R.M, & Medina M.W. (2019). Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation. Stem cell research, 37, 101434.
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