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Recombinant shh c25ii

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Recombinant SHH (C25II) is a laboratory product produced by R&D Systems. It is a recombinant form of the Sonic Hedgehog protein, which is involved in various developmental processes. The C25II variant refers to a specific form of the protein.

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Lab products found in correlation

Bovine serum albumin (bsa), by GenScript (2 mentions) Kaad cyclopamine, by Merck Group (2 mentions) Ihh c28ii, by GenScript (2 mentions) N acetyl l cysteine, by Merck Group (2 mentions)

2 protocols using recombinant shh c25ii

1

Expansion and Preparation of Antibodies

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5E1 antibody was expanded in our laboratory (see supplemental material and methods) and prepared in PBS. IgG1 (InVivoMab, BE0083) was diluted in PBS. KAAD-cyclopamine (Millipore) was prepared in DMSO. Recombinant SHH (C25II) (R&D Systems) and IHH (C28II) (Genscript) were prepared in PBS containing 0.1% bovine serum albumin (BSA). N-Acetyl-L-cysteine (NAC) was purchased from Sigma-Aldrich and prepared in PBS for i.p. injection or sterile tap water for supplemented drinking water. For NAC solution, pH was adjusted to 7.4.
Kasiri S., Chen B., Wilson A.N., Reczek A., Mazambani S., Gadhvi J., Noel E., Marriam U., Mino B., Lu W., Girard L., Solis L.M., Luby-Phelps K., Bishop J., Kim J.W, & Kim J. (2020). Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species. Oncogene, 39(16), 3258-3275.
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2

Preparation of Reagents for SHH/IHH Study

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5E1 antibody was expanded in our laboratory (see Supplementary material and methods) and prepared in PBS. IgG1 (InVivoMab, BE0083) was diluted in PBS. KAAD-cyclopamine (Millipore) was prepared in DMSO. Recombinant SHH (C25II) (R&D Systems) and IHH (C28II) (Genscript) were prepared in PBS containing 0.1% bovine serum albumin (BSA). N-Acetyl-L-cysteine (NAC) was purchased from Sigma-Aldrich and prepared in PBS for i.p. injection or sterile tap water for supplemented drinking water. For NAC solution, pH was adjusted to 7.4.
Kasiri S., Chen B., Wilson A.N., Reczek A., Mazambani S., Gadhvi J., Noel E., Marriam U., Mino B., Lu W., Girard L., Solis L.M., Luby-Phelps K., Bishop J., Kim J.W, & Kim J. (2020). Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species. Oncogene, 39(16), 3258-3275.
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