HUVECs and monocytic U937 cells were obtained from Clonetics (Walkersville, MD, USA), and HEK 293T epithelial cells were obtained from the American Type Culture Collection (#CCL-2, Rockville, MD, USA). Endothelial growth medium-2, Dulbecco’s modified Eagle’s medium, fetal bovine serum, and antibiotics were purchased from Gibco (Grand Island, NY, USA). Human tumor necrosis factor-α (TNF-α) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against cell adhesion molecules (i.e., VCAM-1 and ICAM-1) and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX, USA) and Sigma-Aldrich, respectively. An anti-mouse macrophage specific antibody (galectin-3, clone A3A12) was purchased from Abcam (Cambridge, UK). For analysis with the in vivo imaging system (IVIS), ProSense® 750 FAST Fluorescent Imaging Agent was purchased from PerkinElmer (Santa Clara, CA, USA).
Endothelial growth medium 2
Manufactured by Thermo Fisher Scientific
Sourced in United States
Endothelial growth medium-2 is a specialized culture medium designed to support the growth and maintenance of endothelial cells in vitro. It provides the necessary nutrients and growth factors required for the optimal proliferation and differentiation of endothelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Human tumor necrosis factor α, by Merck Group (1 mentions)
Huvecs, by Cambrex (1 mentions)
Hek 293t epithelial cells, by American Type Culture Collection (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Vcam 1, by Santa Cruz Biotechnology (1 mentions)
Icam 1, by Santa Cruz Biotechnology (1 mentions)
Ts100, by Nikon (1 mentions)
2 protocols using endothelial growth medium 2
1
Inflammatory Cell Interaction Assay
2
Quantification of Endothelial Progenitor Cell Tube Formation
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The tube-forming ability of the EPCs was quantified by using Cultrex®In Vitro Angiogenesis Assay Tube Formation Kit (cat. no. 3470-096-K; Trevigen, Inc.) according to the manufacturer's protocols. For Basement Membrane Extract (BME) coating, 50 µl BME solution (contained within the kit) per well was added into the 96-well plate and the plate was incubated at 37˚C for 60 min. In total, 3x104 EPCs were seeded into each well containing gelled BME and incubated in Endothelial Growth Medium-2 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 30 ng/ml FGF-2 (Trevigen, Inc.) at 37˚C for 6 h. The mean number of complete tubes (a tube was defined as a linear sequence of cells linking two nodes) formed by EPCs was counted in five random fields of view under inverted light microscope at x100 magnification (TS100; Nikon Corporation).
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