The LGSC spheres were digested into single cells with 0.05% trypsin for 5 min at 37 °C and were then fixed in 4%PFA for 10 min. The single cells were treated with blocking buffer (PBS, 10% normal non-immune goat serum and 0.01% Triton) for 30 min and were then incubated with diluted primary antibodies Anti-Krt14 (conjugated with FITC, 1:10, Abcam) for 30 min at 4 °C. Finally, the stained cells were measured with a FACS LSR-II Flow Cytometer (BD). The control was an isotype.
Anti krt14
Manufactured by Abcam
Sourced in United Kingdom
Anti-Krt14 is a laboratory reagent that can be used to detect the presence of keratin 14 (Krt14) protein in biological samples. Krt14 is a type I intermediate filament protein that is expressed in basal cells of stratified squamous epithelial tissues. Anti-Krt14 can be used in various research applications, such as immunohistochemistry and Western blotting, to study the expression and localization of Krt14 in cells and tissues.
Automatically generated - may contain errors
2 protocols using anti krt14
1
Immunophenotyping of Keratinocyte Cultures
2
Antibody-Based Protein Detection Assay
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The following antibodies were used: anti-KRT14 (Abcam, UK; Cat. No. ab7800), anti-KRT5 (Abcam, UK; Cat. No. ab52635), anti-RIPK4 (Santa Cruz Biotechnology, USA; Cat. No. sc-83320), anti-GST (Santa Cruz Biotechnology, USA; Cat. No. sc-459), anti-Flag M2 (Sigma-Aldrich, USA; Cat. No. F3165), secondary antibody HRP-conjugated antimouse (Bio-Rad, USA; Cat. No. 170-5047), antirabbit (Bio-Rad, USA; Cat. No. 170-5045), secondary antimouse-Cy3 (Abcam, UK; Cat. No. ab97035), and secondary antirabbit-Alexa Fluor 488 (Abcam, UK; Cat. No. ab150061).
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