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Click it plus edu flow cytometry assay kit

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The Click-iT Plus EdU Flow Cytometry Assay Kit is a laboratory equipment product designed for cell proliferation analysis using flow cytometry. It enables the detection and quantification of newly synthesized DNA in proliferating cells.

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Lab products found in correlation

Ly6g pe, by BD (2 mentions)

Close spelling variants of this product

Flow cytometry assay Flow cytometry ITS Plus

2 protocols using click it plus edu flow cytometry assay kit

1

Tracking Lung Cell Proliferation After Irradiation

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Mice were treated with a single intraperitoneal injection of EdU dissolved in PBS (25 mg/kg body weight). Mice received targeted lung irradiation 1h later, and were culled either 1h or 7 days post-irradiation. Lungs and bone marrow were harvested, and EdU incorporation assessed in single cell suspensions stained for Ly6G-PE (BD Biosciences, clone 1A8) using the Click-iT Plus EdU Flow Cytometry Assay Kit. Data was acquired on a BD LSR-Fortessa and analysed using FlowJo 10.4.2.
Nolan E., Bridgeman V.L., Ombrato L., Karoutas A., Rabas N., Sewnath C.A., Vasquez M., Rodrigues F.S., Horswell S., Faull P., Carter R, & Malanchi I. (2022). Radiation exposure elicits a neutrophil-driven response in healthy lung tissue that enhances metastatic colonization. Nature cancer, 3(2), 173-187.
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2

Tracking Lung Cell Proliferation After Irradiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were treated with a single intraperitoneal injection of EdU dissolved in PBS (25 mg/kg body weight). Mice received targeted lung irradiation 1h later, and were culled either 1h or 7 days post-irradiation. Lungs and bone marrow were harvested, and EdU incorporation assessed in single cell suspensions stained for Ly6G-PE (BD Biosciences, clone 1A8) using the Click-iT Plus EdU Flow Cytometry Assay Kit. Data was acquired on a BD LSR-Fortessa and analysed using FlowJo 10.4.2.
Nolan E., Bridgeman V.L., Ombrato L., Karoutas A., Rabas N., Sewnath C.A., Vasquez M., Rodrigues F.S., Horswell S., Faull P., Carter R, & Malanchi I. (2022). Radiation exposure elicits a neutrophil-driven response in healthy lung tissue that enhances metastatic colonization. Nature cancer, 3(2), 173-187.
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