Sureselect human all exon v6 capture kit

North African samples from two Tunisian populations were sequenced in the present study: Amazigh individuals from two villages in the Tataouine governorate in southern Tunisia (n = 20) that were pooled together because of their geographical closeness (~ 20 km apart), and non-Amazigh individuals from the city of Tunis (n = 55). Participants were healthy volunteers with appropriate informed consent. Amazigh individuals were Chela-speakers, a language in the Tamazight family, while non-Imazighen were Arab-speakers. Blood samples were collected for non-related individuals in these populations and whole-exome sequencing was performed with the Agilent SureSelect Human All Exon V6 capture kit.

Whole exome sequencing was performed by Macrogen, with data uploaded to the University of Southampton supercomputer Iridis5 in February 2021. Agilent SureSelect Human All Exon V6 capture kit was used for all 28 samples.

We used the CollectHsMetrics command from Picard (v.2.23.8) with default parameters to retrieve the number of mapped reads in targeted regions, near them, or somewhere else. The command was run for the 194 samples (172 + 22) described above, which were also sequenced using the Twist Exome 2.0 panel (kit 3) and Illumina NovaSeq 6000 machines with at least 12 Gb of output per sample. Additionally, the same command was run on 51 samples sequenced using the SureSelect Human All Exon V6 capture kit by Agilent (sequenced on a NovaSeq 6000), as well as 9 samples sequenced using the TruSight One Expanded capture kit by Illumina and sequenced with a NextSeq 500 system (Illumina).