Infinite f200 pro multimodal microplate reader

Streptozotocin (STZ), D-galactose, protease inhibitor cocktail, PhosStop phosphatase inhibitor tablets and DPP-IV inhibitor were acquired from Sigma-Aldrich Chemie (Munich, Germany). The glucose measuring kit (Greiner Diagnostic Glucose GOD-PAD-PAP) was acquired from Dijagnostika (Sisak, Croatia). The Amplex Red Galactose/Galactose Oxidase Kit was purchased from Invitrogen (Eugene, OR, USA). The ELISA Kits for rat/mouse insulin, active GLP-1 and total GLP-1 were acquired from Merck Millipore (Billerica, USA).
The following antibodies were used: anti-GLP-1R / Santa Cruz Biotechnology (Santa Cruz, CA, USA), monoclonal anti-β-actin / Sigma-Aldrich (St. Louis, Missouri, USA) as well as anti-mouse IgG horseradish peroxidase-linked antibody and anti-rabbit IgG horseradish peroxidase-linked antibody acquired from CellSignaling (Beverly, MA, USA). The chemiluminescent Western blot detection kit (SuperSignal West Femto Chemiluminescent Substrate) was acquired from Thermo Scientific (Rockford, IL, USA). The Anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 was acquired from Thermo Fisher Scientific (Waltham, MA, USA) and donkey serum from Sigma-Aldrich Chemie (Munich, Germany).
Colourimetric and fluorimetric measurements were performed on an Infinite F200 PRO multimodal microplate reader (Tecan, Switzerland).