Therapeutic anti-CD8+ (clone 2.43), anti-CD4+ (clone GK1.5), and anti-NK1.1 (clone PK136), antibodies were produced by BioXcell. Antibodies used for flow cytometry were purchased from the following sources (dilutions are indicated in parentheses): eBioscience (CD45.2 Alexa Fluor 700, cat. 56-0454 [1:200]; CD3 PE-Cy7, cat. 25-0031 [1:200]; CD4 ef450, cat. 48-0041 [1:200]; CD4 APC-efluor780, cat. 47-0041 [1:400]; CD8 PerCP-efluor710, cat. 46-0083 [1:200]; CD11b APC-efluor 780, cat. 47-0112 [1:600]; NK 1.1 PE, cat. 12-5941 [1:200]; FoxP3 APC, cat. 17-5773 [1:200]); and Invitrogen (Granzyme B PE-Texas red, cat. GRB17 [1:125]).
Granzyme b pe texas red
Manufactured by Thermo Fisher Scientific
Sourced in Canada
Granzyme B PE-Texas Red is a fluorescently labeled antibody targeting the Granzyme B protein. Granzyme B is a serine protease involved in the cytotoxic T-cell and natural killer cell-mediated apoptosis pathway. The PE-Texas Red conjugate provides a fluorescent signal that can be detected and analyzed using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti nk1.1 clone pk136, by BioXCell (1 mentions)
Cd45.2 alexafluor700, by Thermo Fisher Scientific (1 mentions)
Ccr7 bv711, by BioLegend (1 mentions)
Ifn γ percp cy5, by BD (1 mentions)
Cd27 pecy5, by Thermo Fisher Scientific (1 mentions)
Cd19 pacificblue, by BD (1 mentions)
Cd4 pe cy5, by BD (1 mentions)
Cd45ro af700, by BD (1 mentions)
Cd16 pacific blue, by BD (1 mentions)
Cd3 apc h7, by BD (1 mentions)
Cd8 bv570, by BioLegend (1 mentions)
Tnf α af647, by BioLegend (1 mentions)
Cd14 pacific blue, by BD (1 mentions)
Cd107a pe cy7, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
2 protocols using granzyme b pe texas red
1
Multiparametric Immune Cell Analysis
2
Intracellular Cytokine Staining Workflow
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Intracellular cytokine staining was performed as previously described(1) using the following markers: CD3-APCH7, CD107a PECy7, CD14-Pacific Blue, CD16-Pacific Blue, CD4-PECy5.5, IFN-γ-PerCPCy5.5, CD45RO-AF700, CD19-Pacific Blue (BD Biosciences, San Jose, CA), TNFα-AF647, CD8-BV570, CCR7-BV711 (BioLegend, San Diego, CA), granzyme B-PE Texas Red (Invitrogen), CD27-PECy5 (eBioscience, San Diego, CA) perforin-FITC(Abcam, Cambridge, UK). Prepared cells were acquired using an LSR II flow cytometer equipped with BD FACSDiva software (BD Biosciences). Acquired data was analyzed using the FlowJo software version 7.6.3 (Tree Star).
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