At 101

PC-9 gefitinib-resistant cells (PC-9-GR) was kindly provided by Dr. Bing Xia at Hangzhou Cancer Hospital (Hangzhou, China) [16 (link)]. H1975 cell lines was obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in standard RPMI-1640 media (Invitrogen, Carlsbad, CA, USA) supplemented with 10 % heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA, USA), 2 mM l-glutamine, and 1 % penicillin/streptomycin at 37 °C in a humidified incubator with 5 % CO₂. AT-101, ABT-263, and gefitinib were purchased from Sigma (St. Louis, MO, USA). Both reagents were dissolved in dimethyl sulfoxide (DMSO), stored at−80 °C, and diluted in culture medium for described experiments.

All LDH inhibitors (AT-101 (Catalog #SML0433, ≥98%), FX-11 (Catalog #427218, ≥96%), galloflavin (Catalog #SML0776, ≥95%), gossypol (Catalog #G8761, ≥95%), GSK2837808A (Catalog #5.33660, ≥95%), isosafrole (Catalog #329606, ≥100%), sodium oxamate (Catalog #02751, ≥98%), stiripentol (Catalog #S6826, ≥98%), and NHI-2 (Catalog #SML1463, ≥98%)) were purchased from Sigma-Aldrich (Burlington, MA, USA).