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Cyquant mtt cell proliferation assay

Manufactured by Thermo Fisher Scientific
Sourced in United States

The CyQUANT MTT cell proliferation assay is a colorimetric method used to quantify cell proliferation. It measures the activity of cellular enzymes that reduce the tetrazolium dye MTT to formazan, producing a purple color. The intensity of the color is proportional to the number of viable cells.

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2 protocols using cyquant mtt cell proliferation assay

1

Cell Viability Measurement Protocols

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HepaRG and HFF cell viabilities were measured using an alamarBlue assay (Bio-Rad) following the manufacturer’s protocol. 17Cl-1 cell and BMDM viabilities were measured using the CyQUANT MTT cell proliferation assay (Thermo Fisher Scientific) following the manufacturer’s protocol. Means and standard deviations were calculated from at least three independent experiments.
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2

FOXO1 Inhibitor Cytotoxicity Assay

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HGECs (5 × 103 cells) were seeded into 96-well plates. After adhesion, cells were treated with different concentrations of FOXO1 inhibitor (Fi) AS1842856 (0 μM to 100 μM) for 24 h. The cell viability was evaluated using a testing kit (CyQUANT™ MTT Cell Proliferation Assay, Thermo Fisher Scientific Inc., Waltham, MA, USA).
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