Human epcam duoset elisa kit

Retrovirus transduced MDCK or transiently transfected HEK-293T cells with EpCAM-GFP constructs were lightly trypsinized after 48 hours and plated on a glass bottom 35 mm dish with serum-free Opti-MEM media (ThermoFisher Scienti c, Grand Island, New York). Cells were visualized and captured with a uorescence microscope (EVOS digital inverted microscope at 20X or 40X magni cations). Duplicate cell culture was used for data measurements on a Zeiss LSM 880 microscope equipped with the AiryScan detector, an Argon laser (Melles-Griot) for 488 nm excitation and a Zeiss Plan-Apochromat 63×/1.4 NA DIC M27 Oil objective. The microscope is equipped with temperature and CO 2 controls that were kept at 37 °C and 5%, respectively.
EpCAM ELISA HEK-293T transiently transfected cells or tumor cell lines were plated in serum free Opti-MEM media.
After 48 hours, conditioned media was used as a source of secreted/soluble EpCAM. Human EpCAM DuoSet ELISA kit (DY960) was used from R&D Systems Inc. (Minneapolis, MN) as recommended.