Nsc35 c

Manufactured by MikroMasch

Sourced in Bulgaria

The NSC35-C is a scanning probe microscope component designed for high-resolution surface analysis. It provides a stable and precise platform for mounting various microscope probes. The core function of the NSC35-C is to enable the measurement and characterization of surface topography and properties at the nanoscale level.

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Lab products found in correlation

Mfp 3d bio afm, by Oxford Instruments (2 mentions)

2 protocols using nsc35 c

Nanofiber Scaffold Stiffness Characterization

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All AFM measurements were performed with a MFP-3D Bio-AFM (Asylum Research, Santa Barbara, CA, United States). Nanofiber scaffolds were prepared as described and fixed on glass slides with double-sided tape. For stiffness measurements, 9.6 μm spherical PMMA particles (modulus ∼3 GPa) were attached to tipless silicon cantilevers (NSC35-C, Mikromasch, Sofia, Bulgaria) using a two-part epoxy and dried for at least 24 h. We use the thermal equipartition method (Hutter and Bechhoefer, 1993 (link)) to calibrate the cantilever spring constant immediately prior to use by indenting a glass substrate and performing a Lorentzian fit to the thermal spectrum. Topological images and accompanying stiffness characterizations of nanofibers were obtained from regions of 80 × 80 μm² force maps with 96 × 96 force curves per image. All the force-distance measurements were performed with 2 μm/sec approach velocity using a 500 nN force trigger. Force-distance plots were transformed into force-indentation depth plots and then 10–100% indentation depth was used to evaluate Young’s modulus using a Hertzian contact model.

San Emeterio C.L., Hymel L.A., Turner T.C., Ogle M.E., Pendleton E.G., York W.Y., Olingy C.E., Liu A.Y., Lim H.S., Sulchek T.A., Warren G.L., Mortensen L.J., Qiu P., Jang Y.C., Willett N.J, & Botchwey E.A. (2021). Nanofiber-Based Delivery of Bioactive Lipids Promotes Pro-regenerative Inflammation and Enhances Muscle Fiber Growth After Volumetric Muscle Loss. Frontiers in Bioengineering and Biotechnology, 9, 650289.

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Measuring Nanofiber Scaffold Stiffness by AFM

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All AFM measurements were performed with a MFP-3D Bio-AFM (Asylum Research, Santa Barbara, CA, USA). Nanofiber scaffolds were prepared as described and fixed on glass slides with double-sided tape. For stiffness measurements, 9.6μm spherical PMMA particles (modulus ~ 3 GPa) were attached to tipless silicon cantilevers (NSC35-C, Mikromasch, Sofia, Bulgaria) using a two-part epoxy and dried for at least 24 hours. We use the thermal method (43 (link)) to calibrate the cantilever spring constant immediately prior to use by indenting a glass substrate and performing a Lorentzian fit to the thermal spectrum. Topological images and accompanying stiffness characterizations of nanofibers were obtained from regions of 80×80 μm² force maps with 96×96 force curves per image. All the force-distance measurements were performed with 2 um/sec approach velocity using a 500 nN force trigger. Force-distance plots were transformed into force-indentation depth plots, then 10–100% indentation depth was used to evaluate Young’s modulus using a Hertzian contact model.

Hymel L.A., Ogle M.E., Anderson S.E., San Emeterio C.L., Turner T.C., York W.Y., Liu A.Y., Olingy C.E., Sridhar S., Lim H.S., Sulchek T., Qiu P., Jang Y.C., Willett N.J, & Botchwey E.A. (2020). MODULATING LOCAL S1P RECEPTOR SIGNALING AS A REGENERATIVE IMMUNOTHERAPY AFTER VOLUMETRIC MUSCLE LOSS INJURY. Journal of biomedical materials research. Part A, 109(5), 695-712.

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