Cfi plan apo lambda 20

Lung tissues were collected from mice that had been sacrificed under a CO₂ atmosphere. They were filled with PBS (−):optimal cutting temperature (OCT) compound (1:1), and the resulting lung tissues were embedded in OCT compound. The resulting tissues were sliced at a thickness of 5 μm. After placing the slices on MAS-coated glass slides (Matsunami, Tokyo, Japan), they were then incubated with the first antibody solution using a Cy3-labeled anti-αSMA antibody (Sigma-Aldrich, C6198), anti-mouse CD31 Armenian Hamster immunoglobulin G (IgG) (MA31505, Thermo Fisher Scientific), anti-mouse VEcad rat IgG (138001, BioLegend), and anti-bromodeoxyuridine (BrdU) mouse IgG (364101, BioLegend). The slices were then washed twice with PBS (−) and immersed in a second antibody solution containing DyLight488-labeled anti-Armenian hamster IgG goat IgG (405503, BioLegend) for CD31 and AlexaFluor647-labeled anti-Rat IgG goat IgG (A21247, Thermo Fisher Scientific) for 30 min. The slices were covered with a coverslip (Matsunami, 1s) after washing with PBS (−). The tumor slices were observed with a Nikon A1R system (Nikon, Tokyo, Japan) using CFI Plan Apo Lambda 20× or CFI Plan Apo VC 60× water immersion objective lens.