Eds detector

Manufactured by Bruker

The EDS detector is a core component of analytical instruments used in materials analysis. It is designed to detect and measure the energy of X-rays emitted from a sample, enabling the identification and quantification of the elements present. The EDS detector plays a crucial role in providing elemental information to support various applications, including materials science, geology, and forensics.

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Lab products found in correlation

Mira3 fesem, by TESCAN (2 mentions) Empyrean x ray diffractometer, by Malvern Panalytical (1 mentions)

4 protocols using eds detector

Morphological and Elemental Analysis of DCIS

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After all analysis was complete SEM/EDS was used to verify the morphological and elemental findings and to take higher resolution images of calcifications. Samples were carbon coated (~15 nm thick) and analyzed in a Mira3 FESEM (Tescan, Czech Republic). High resolution images were acquired at 5 keV (working distance 3 mm) with the in-beam secondary electron detector and/or the in-beam backscatter detector. EDS was carried out at 20 keV (working distance 15 mm) using the EDS detector (Bruker). EDS maps were acquired for at least 5 minutes and a minimum of 4 particles were examined from each DCIS region. The Esprit EDS software was used to generate maps and analyze EDS data.

Kunitake J.A., Choi S., Nguyen K.X., Lee M.M., He F., Sudilovsky D., Morris P.G., Jochelson M.S., Hudis C.A., Muller D.A., Fratzl P., Fischbach C., Masic A, & Estroff L.A. (2017). Correlative imaging reveals physiochemical heterogeneity of microcalcifications in human breast carcinomas. Journal of structural biology, 202(1), 25-34.

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SEM and EDS Analysis of Powdered Samples

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SEM images were obtained with a Hitachi SU8230 field emission scanning electron microscope. The acceleration voltage was set to 2.5 kV and the probe current to 10 μA. In order to reduce sample charging, 1.5 kV deceleration voltage was applied, resulting in an effective voltage (“landing voltage”) of 2.5–1.5 = 1 kV. The same instrument was equipped with a Bruker EDS detector. Samples were prepared as powders or pellets attached to carbon tape. The working distance was 15 mm for EDS analysis, and the scanned area was ca. 1000 μm². The accelerating voltage was set to 10 kV so that both zirconium (Lα = 2.042 keV) and ruthenium (Lα = 2.558 keV) could be reliably quantified.

Thoresen E.M., Øien-Ødegaard S., Kaur G., Tilset M., Lillerud K.P, & Amedjkouh M. (2020). Strongly visible light-absorbing metal–organic frameworks functionalized by cyclometalated ruthenium(ii) complexes. RSC Advances, 10(15), 9052-9062.

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Mineral Formation in Microbial Cultures

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The formed minerals were recovered from pure cultures of the mineral-forming strains as described by Al Disi et al., (2017) . The recovered minerals were analyzed by SEM/EDS and XRD.
SEM images were obtained using Nova Nano Scanning Electron Microscope equipped with Bruker EDS Detector with five nm resolution and a magnification of 200,000×.
The bulk mineralogical composition of the recovered minerals was determined using a PANalytical-multipurpose Empyrean X-ray diffractometer. The Mg mol% of carbonate minerals were calculated according to the position of the XRD (d104) peak of high magnesium calcite using the formula of Goldsmith et al. (1961) .

Abdelsamad R., Al Disi Z., Abu-Dieyeh M., Al-Ghouti M.A, & Zouari N. (2022). Evidencing the role of carbonic anhydrase in the formation of carbonate minerals by bacterial strains isolated from extreme environments in Qatar. Heliyon, 8(10), e11151.

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Cryo-SEM Analysis of Spheroid Samples

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The cryo sections were carbon coated and analyzed in a Mira3 FESEM (Tescan, Czech Republic). High resolution images were acquired at 5 keV at working distance 3 mm with the in-beam secondary electron detector and the in-beam backscatter detector. EDS was carried out at 10 keV at working distance 15 mm using Bruker EDS detector. For SEM imaging and EDS analysis, EDS maps were acquired from at least three regions within 3 spheroid sections and a minimum of 5 particles was examined from each region. For each map, the acquisition time was 12 minutes to ensure adequate counts for calcium signal. The Esprit EDS software was used to generate maps and analyze EDS data and OriginPro 9.1 was used for data plotting. Count intensities were normalized based on the Ca peak intensity.

Vidavsky N., Kunitake J.A., Chiou A.E., Northrup P.A., Porri T., Ling L., Fischbach C, & Estroff L.A. (2018). Studying biomineralization pathways in a 3D culture model of breast cancer microcalcifications. Biomaterials, 179, 71-82.

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