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2 protocols using mouse anti mavs e 3

1

Antibodies and Reagents for Immunological Assays

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Rabbit anti-DYKDDDDK tag (D6W5B), rabbit anti-RIG-I (D14G6), rabbit anti-IRF3 (D83B9), rabbit anti-pIRF3 (4D46), rabbit anti-TBK1 (3031S), rabbit anti-pTBK1 (D52C2), and rabbit anti-TRAF3 antibodies (Abs) were obtained from Cell Signaling Technology (USA); the mouse anti-MAVS (E-3) and mouse anti-TRAF3 (G-6) antibodies were obtained from Santa Cruz Biotechnology (USA); the mouse anti-actin and rabbit anti-calnexin antibodies were obtained from Proteintech (Wuhan, China); the mouse anti-Flag M2 antibody was obtained from Sigma-Aldrich (USA); the mouse anti-Myc (9E10) antibody was obtained from Origene (USA); the rabbit anti-GM130 antibody was obtained from Abcam (United Kingdom); the mouse anti-GAPDH antibody (AF0006) was obtained from Beyotime (China); and the mouse anti-HA antibody was obtained from MDL Biotech (China). Alexa Fluor 488 goat anti-rabbit IgG secondary antibody, Alexa Fluor 568 goat anti-mouse IgG secondary antibody, Alexa Fluor 488 goat anti-mouse IgG secondary antibody, and Alexa Fluor 568 goat anti-rabbit IgG secondary antibody were obtained from Thermo Fisher Scientific. Protein A/G beads were obtained from Santa Cruz Biotechnology, and anti-Flag magnetic beads were obtained from Bimake (USA). Recombinant proteins of human IFN-β (P5660) and human IFN-λ1 (P5669) were purchased from Beyotime (China).
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2

Antibodies for Influenza Virus Protein Detection

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Commercial primary antibodies used in this study included mouse anti-Flag (M2; Sigma-Aldrich), mouse anti-V5 (Invitrogen), mouse anti-Myc (9E10; Santa Cruz), mouse anti-HA (F7; Santa Cruz), mouse anti-GAPDH (6C5; Santa Cruz), mouse anti-β-actin (Sigma-Aldrich), mouse anti-α-tubulin (Sigma-Aldrich), mouse anti-MAVS (E3; Santa Cruz), rabbit anti-MAVS (D5A9E; Cell Signaling), rabbit anti-COXIV (3E11; Cell Signaling) and rabbit anti-PA (PA5-32224; Invitrogen). Mouse monoclonal antibody to PB1-F2 was raised using the custom antibody production service of Genscript (Piscataway, New Jersey) with full-length recombinant protein of PB1-F2 of A/Zhejiang/DTID-ZJU01/2013 (H7N9) expressed from E. coli as immunogen.
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