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Dylight800 goat anti mouse igg

Manufactured by Rockland Immunochemicals
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Sourced in United Kingdom

DyLight800-goat anti-mouse (GAM) IgG is a fluorescently labeled secondary antibody. It is designed to detect and bind to mouse immunoglobulin G (IgG) for use in various immunoassays and imaging applications.

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Lab products found in correlation

Odyssey system, by LI COR (2 mentions) Anti c myc 9e10, by Abcam (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti c myc mab clone 9e10, by Abcam (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Iblot 2 dry blotting system, by Thermo Fisher Scientific (1 mentions)

2 protocols using dylight800 goat anti mouse igg

1

Western Blot Immunodetection of Protein Tags

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were separated under reducing conditions on 10–20% Tris-glycine gels and transferred onto nitrocellulose membranes and probed with either anti-c-myc (9E10, cat#ab206486, Abcam) or anti-FLAG (M2, cat#F480, Sigma-Aldrich) mAbs (1 µg/ml), followed by incubation with DyLight800-goat anti-mouse (GAM) IgG (1:5000 dilution) (cat#610-145-121, Rockland Immunochemicals). Visualization of protein bands was performed with the Odyssey® system (LI-COR Biosciences).
Compte M., Harwood S.L., Muñoz I.G., Navarro R., Zonca M., Perez-Chacon G., Erce-Llamazares A., Merino N., Tapia-Galisteo A., Cuesta A.M., Mikkelsen K., Caleiras E., Nuñez-Prado N., Aznar M.A., Lykkemark S., Martínez-Torrecuadrada J., Melero I., Blanco F.J., Bernardino de la Serna J., Zapata J.M., Sanz L, & Alvarez-Vallina L. (2018). A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nature Communications, 9, 4809.
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2

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were separated under reducing conditions on 12% Tris-glycine gels and transferred to a nitrocellulose membrane using iBlot 2 Dry Blotting System (Life Technologies). The membrane was blocked with Odyssey Blocking Buffer (LI-COR Biosciences, Lincoln, NE, USA) and probed with anti-c-myc mAb (clone 9E10, Abcam, Cambridge, UK), followed by incubation with DyLight800-goat anti-mouse (GAM) IgG (Rockland Immunochemicals, Gilbertsville, PA, USA).Visualization of protein bands was performed with the Odyssey® system (LI-COR Biosciences).
Mikkelsen K., Harwood S.L., Compte M., Merino N., Mølgaard K., Lykkemark S., Alvarez-Mendez A., Blanco F.J, & Álvarez-Vallina L. (2019). Carcinoembryonic Antigen (CEA)-Specific 4-1BB-Costimulation Induced by CEA-Targeted 4-1BB-Agonistic Trimerbodies. Frontiers in Immunology, 10, 1791.
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