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G6pdi 1 31484

Manufactured by Cayman Chemical
Sourced in Estonia, Germany

The G6PDi-1 (31484) is a laboratory reagent that functions as a glucose-6-phosphate dehydrogenase inhibitor. It is used for research purposes in a controlled laboratory setting.

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2 protocols using g6pdi 1 31484

1

DMEM-based Cell Culture Protocol

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Dulbecco’s modified Eagle’s medium (DMEM with 25 mM glucose) powder (12100061) and penicillin G/streptomycin sulfate (15140122) solution was from Thermo Fisher Scientific (Schwerte, Germany; RRID:SCR_008452). Fetal calf serum (FCS; F7524) was purchased from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). G6PDi-1 (31484) was obtained from Cayman Chemical (Tallinn, Estonia; RRID:SCR_008945), 6AN (A-0630) from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988), DHEA (30770) from Fluka (Buchs, Switzerland), β-lapachone (ab141097) from Abcam (Berlin, Germany; RRID:SCR_012931), ES936 (sc-362737) from Santa Cruz Biotechnology (Heidelberg, Germany; RRID:SCR_008987) and WST-1 (W201) from Dojindo (Munich, Germany). All enzymes for the assays to quantify lactate, glucose and glutathione were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other chemicals were obtained in the highest purity available from Merck (Darmstadt, Germany; RRID:SCR_001287), Fluka (Buchs, Switzerland), Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988), AppliChem (Darmstadt, Germany; RRID:SRC_005814) or Carl Roth (Karlsruhe, Germany; RRID:SCR_005711). Sterile cell culture material as well as non-sterile cups and microtiter plates were from Sarstedt (Nümbrecht, Germany).
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2

Detailed Cell Metabolite Assay Protocol

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Dulbecco’s modified Eagle’s medium (DMEM with 25 mM glucose) powder and penicillin/streptomycin solution was obtained from Gibco/Invitrogen (Darmstadt, Germany) and fetal calf serum (FCS) from Sigma-Aldrich (Steinheim, Germany). β-lapachone (ab141097) was purchased from Abcam (Berlin, Germany), dicoumarol (M1390) and iodoacetate (I6375) were from Sigma-Aldrich (Steinheim, Germany). ES936 (sc-362737) was obtained from Santa Cruz Biotechnology (Heidelberg, Germany), WST1 (W201) from Dojindo (Munich, Germany) and G6PDi-1 (31484) from Cayman Chemical (Tallinn, Estonia). The enzymes for the metabolite assays were purchased from Roche Diagnostics (Mannheim, Germany). All other chemicals of the highest purity available were purchased from Merck (Darmstadt, Germany), Sigma-Aldrich (Steinheim, Germany) or AppliChem (Darmstadt, Germany). Sterile cell culture material and non-sterile microtiter plates were from Sarstedt (Nümbrecht, Germany).
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