Nsc15 al bs

Bacteria were mounted on gelatin-coated mica. For each of the control and treated bacteria, a cocktail consisting of two strains from each bacterial culture was prepared. For the oil-treated bacteria, 1 ml of each bacterial culture in MHB (OD: 0.08–0.13 at 600 nm) was mixed with 1 ml of the oil treatments at a concentration of 10 µl/ml. The tubes were then incubated at 37 °C for 24 h. After incubation, the tubes were centrifuged (4500 rpm for 10 min) and the pellet was washed with sterilized distilled water. The tubes were centrifuged again, the supernatant was discarded, and the pellet was mixed with 50 µl distilled water and vortexed. From this solution, 20 µl was mounted on a mica slide, left to dry, washed with distilled water, and left to dry. The slides were then prepared for imaging. For the control, bacteria were added to sterilized distilled water (OD: 0.5–1.0 600 nm), followed by the same process. All bacteria in this study were imaged, except for L. monocytogenes, as the oil showed no significant effect on bacterial cells. Imaging of the bacterial cells was performed using the AFM non-contact mode (QScan Sync SP mode, AIST-NT), n-type silicon tip (NSC15/Al BS, Mikromasch), nominal spring constant of 40 N/m, resonance frequency of 356 kHz, and scanning rate of 0.3–0.7 Hz. Images were analyzed using the Gwyddion Software.