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Anti cenpe

Manufactured by Abcam

Anti-CENPE is a primary antibody that recognizes the centromere protein E (CENPE), a kinesin-like protein involved in chromosome movement and segregation during cell division. It can be used for applications such as Western blotting, immunohistochemistry, and immunocytochemistry.

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2 protocols using anti cenpe

1

Cell Cycle Regulation Protein Antibodies

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The detailed procedure was described
in our previous study.54 (link),55 (link),58 (link),59 (link) Primary antibodies, anti-CENPA, anti-CENPE,
anti-CENPF, and anti-CENPI antibodies, were purchased from abcam (Cambridge,
U.K.); anti-CDK1 and anticyclin B1 antibodies were purchased from
Santa Cruz Biotechnology, Inc. (Dallas, TX); and anti-EZH2 and anti-H3K27me3 antibodies were purchased from GeneTex International Corporation
(HsinChu, Taiwan), anti-Caspase-3 was purchased from GeneTex International
Corporation; anti-cleaved Caspase-3 was purchased from Cell Signaling
Technology (Danvers, Massachusetts).
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2

Immunofluorescence and Western Blot Antibodies

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Anti-53BP1 was the mouse monoclonal from Bethyl. Anti-CENP-E was mouse monoclonal [1H12] from Abcam was used for patient LCL indirect immunofluorescence whilst Anti-CENP-E (AKIN04) from Cytoskeleton was used for Western blotting. Anti-BUBR1 was a mouse monoclonal (ab4637) was from Abcam. Anti-α-tubulin was a mouse monoclonal (clone B512) from Sigma-Aldrich and anti-β-tubulin was a rabbit polyclonal (H-235) from Santa Cruz. Anti-PARP (MCS 1522G) was from AbD Serotec and anti-p85-PARP (cleaved-PARP: G7341) was obtained from Promega
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