Mdpv hydrochloride

Methylone hydrochloride, MDPV hydrochloride, (±) mephedrone hydrochloride, and (±) MDMA hydrochloride were obtained from the NIDA Research Resources Drug Supply Program. (+) methamphetamine hydrochloride, D-amphetamine sulfate, MPTP hydrochloride, DA, polyclonal antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and all buffers and HPLC reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid protein assay kits were obtained from Pierce (Rockford, IL, USA). Polyclonal antibodies against rat tyrosine hydroxylase (TH) were produced as described previously (Kuhn and Billingsley 1987 (link)). Monoclonal antibodies against rat DAT were generously provided by Dr Roxanne A. Vaughan (University of North Dakota, Grand Forks, ND, USA). Polyclonal antibodies against glial fibrillary acidic protein (GFAP) were obtained from Thermo Scientific (Rockford, IL, USA). IRDye secondary antibodies for Odyssey Imaging Systems were purchased from LiCor Biosciences (Lincoln, NE, USA).

MDPV hydrochloride and mecamylamine hydrochloride were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). MDPV and mecamylamine were dissolved in sterile saline (0.9% sodium chloride). Mecamylamine was administered subcutaneously (sc) in a volume of 1 ml/kg body weight. Drug doses are expressed as salt.