Maternal blood was collected in a S-Monovette 7.5 ml serum-gel tubes (Sarstedt, Nümbrecht, Germany) during a routine blood withdrawal the day after delivery, centrifuged, and stored long-term at -80°C until further analysis. Cord blood was also collected in a S-Monovette 7.5 ml serum-gel tubes (Sarstedt, Nümbrecht, Germany) shortly after delivery, centrifuged, and stored at -80°C.
Serum uric acid values were measured photometrically with a Cobas 6000 from Roche Diagnostics (Mannheim, Germany, with reagent uricase, enzymatic test, coefficient of variation (CV) 1.3%). This method is standardized against ID/MS. Cystatin C was determined by immunonephelometry on a Behring Nephelometer (BN) II (Siemens, Erlangen, Germany) (CV low concentrations 3.3, high conc. 2.0%). Adiponectin and leptin concentrations in maternal and cord blood serum were determined by a commercially available ELISA (R&D Systems, Wiesbaden, Germany), high sensitivity CRP (hs-CRP, Immunodiagnostik AG, Bensheim, Germany, CV low conc. 5.4%, high conc. 4.0%) and retinol-binding protein 4 (RBP4), measured by immunonephelometry on a BNII (Siemens, Erlangen, Germany, CV 4.3%). HbA1c, HDL, triglyceride and insulin were measured according to routine methods in the university laboratory.
Serum uric acid values were measured photometrically with a Cobas 6000 from Roche Diagnostics (Mannheim, Germany, with reagent uricase, enzymatic test, coefficient of variation (CV) 1.3%). This method is standardized against ID/MS. Cystatin C was determined by immunonephelometry on a Behring Nephelometer (BN) II (Siemens, Erlangen, Germany) (CV low concentrations 3.3, high conc. 2.0%). Adiponectin and leptin concentrations in maternal and cord blood serum were determined by a commercially available ELISA (R&D Systems, Wiesbaden, Germany), high sensitivity CRP (hs-CRP, Immunodiagnostik AG, Bensheim, Germany, CV low conc. 5.4%, high conc. 4.0%) and retinol-binding protein 4 (RBP4), measured by immunonephelometry on a BNII (Siemens, Erlangen, Germany, CV 4.3%). HbA1c, HDL, triglyceride and insulin were measured according to routine methods in the university laboratory.