Miridian micro rna

HMEC cells were transfected with 200 nM negative control mimic (miRIDIAN micro RNA, Dharmacon), an inhibitor negative control (miRIDIAN micro RNA, Dharmacon), 200 nM miR-19a mimic (hsa-miR-19a-3p, HMI0344, MISSION miRNA mimic, Sigma), 200 nM anti-miR-19a (hsa-miR-19a-3p inhibitor, HSTUD0343, MISSION, Sigma) or a miR-126 mimic (has-miR-126-3p, MISSION miRNA mimic, Sigma) using the siRNA transfection reagent interferin (VWR) according to manufacturer’s protocol. 24 h post transfection cells were starved in MCBD 131 medium (Gibco) for 2 h and then stimulated with 10 ng/mL TNF-α for 2 h for gene expression analysis and 6 h for protein expression of the TF splice variants. THP-1 cells were stimulated with LPS (10 µg/mL) and miR expression and TF activity was assessed.