Pa1 41331

Manufactured by Agilent Technologies

The PA1-41331 is a lab equipment product from Agilent Technologies. It serves as a core component for analytical tasks, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (6 mentions) Axio observer inverted microscope, by Zeiss (3 mentions) Alexa fluor 555, by Thermo Fisher Scientific (3 mentions)

4 protocols using pa1 41331

Tubulin Dynamics in Neuro2a Cells

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Neuro2a cells were seeded at a density of 50 000
cells on glass cover slips. The cells were treated with various concentrations
of TB (0.5, 5, and 50 μM) and incubated overnight at 37 °C.
Similarly, another set of cells treated with various concentrations
of TB were irradiated with red light for 10 min and incubated at 37
°C. The cells were fixed with absolute methanol for 20 min at
−20 °C. After fixation, cells were permeabilized by 0.2%
Triton X-100. After 3 subsequent washes of PBS, the cells were incubated
with 5% horse serum for 1 h. The cells were incubated with the anti-tubulin
(Thermo PA1-41331) and K9JA (Dako A0024) antibody. After overnight
incubation, the cells were incubated with Alexa Fluor 488 (A11034)-
and Alexa Fluor 555 (A32727)-tagged secondary antibodies. The nucleus
was stained with DAPI. The cells were scanned by a Zeiss Axio observer
7.0, apotome 2.0 inverted microscope using 63× magnifications
in oil immersion and at 40% light intensity.

Dubey T., Gorantla N.V., Chandrashekara K.T, & Chinnathambi S. (2019). Photoexcited Toluidine Blue Inhibits Tau Aggregation in Alzheimer’s Disease. ACS Omega, 4(20), 18793-18802.

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Tubulin and K9JA Immunostaining of Neuro2a Cells

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Neuro2a cells (50, 000/well) were seeded on a glass coverslip and allowed to incubate for 24 hours at 37°C. The cells were treated with various concentrations of TB (0.5 and 50 µM) and irradiated for 10 minutes with a red light. After the irradiation, cells were incubated further 24 hours and processed for immunostaining. The treated and untreated cells were fixed with absolute methanol for 20 minutes at -20°C. 0.2% Triton X-100 was used for cell permeabilization. For avoiding the non-specific binding of the antibody, the cells were incubated with 5% horse serum for 1 hour. The cells were incubated with anti-tubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibodies. After overnight incubation, the cells were incubated secondary antibody tagged with Alexa Fluor 488 (Thermo, A11034) (anti-rabbit; dilution 1:1000) and Alexa Fluor 555 (Thermo, A32727) (Anti-mouse; dilution 1:500). The nucleus was stained with DAPI. After the incubation coverslips were mounted using mounting media (70% glycerol) and were sealed on a glass slide. These slides were allowed to air dry at room temperature before the scanning. These samples were scanned on Zeiss Axio observer inverted microscope using 63X magnification in oil emersion and at 40% light intensity.

Chinnathambi S., Dubey T, & Chandrashekar M. (2023). Photo-Excited Toluidine Blue Disaggregates the Repeat Tau and Modulates End-Binding Protein EB1, Cytoskeletal Structure in Neuronal Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 57(2).

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Tubulin and Apoptosis Evaluation in Neuro2a Cells

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Neuro2a cells (50,000/well) were seeded on a glass coverslip and allowed to incubate for 24 hours at 37°C. The cells were treated with various concentrations of TB (0.5 and 50 µM) and irradiated for 10 minutes with a red light. After 24 hours of incubation with PE-TB, the cells were processed for immunostaining. The treated and untreated cells were xed with absolute methanol for 20 minutes at -20°C. 0.2% Triton X-100 was used for cell permeabilization. For avoiding the non-speci c binding of the antibody, the cells were incubated with 5% horse serum for 1 hour. The cells were incubated with antitubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibody. After overnight incubation, the cells were incubated secondary antibody tagged with Alexa Fluor 488 (Thermo, A11034) (anti-rabbit; dilution 1:1000) and Alexa Fluor 555 (Thermo, A32727) (Anti-mouse; dilution 1:500).
The nucleus was stained with DAPI. After the incubation coverslips were mounted using mounting media (70% glycerol) and were sealed on a glass slide. These slides were allowed to air dry at room temperature before the scanning. These samples were scanned on Zeiss Axio observer inverted microscope using 63X magni cation in oil emersion and at 40% light intensity.

Dubey T., Gorantla N.V., & Chinnathambi S. (2020). Photo-excited Toluidine Blue Disaggregates the Repeat Tau and Modulates End-binding Protein EB1, Cytoskeletal Structure in Neuronal Cells.

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Immunostaining of Neuro2a Cells Treated with TB

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Neuro2a cells (50,000/well) were seeded on glass coverslip and allowed to incubate for 24 hours at 37°C. The cells were treated with various concentrations of TB (0.5 and 50 µM) and irradiated for 10 minutes with red light. After 24 hours of incubation with PE-TB, the cells were processed for immunostaining. The treated and untreated cells were fixed with absolute methanol for 20 minutes at -20°C. 0.2% Triton X-100 was used for cell permeabilization. For avoiding the non-specific binding of antibody, the cells were incubated with 5% horse serum for 1 hour. The cells were incubated with anti-tubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibody.
After overnight incubation the cells were incubated secondary antibody tagged with Alexa Fluor 488 (Thermo, A11034) (anti-rabbit; dilution 1:1000) and Alexa Fluor 555 (Thermo, A32727) (Antimouse; dilution 1:500). The nucleus was stain with DAPI. After the incubation coverslips were mounted using mounting media (70% glycerol) and were sealed on a glass slide. These slides were allowed to air dry at room temperature before the scanning. These samples were scanned on Zeiss Axio observer inverted microscope using 63X magnification in oil emersion and at 40% light intensity.

Dubey T., Gorantla N.V., & Chinnathambi S. (2020). Photo-excited Toluidine Blue disaggregates the Repeat Tau and modulates cytoskeletal structure in neuronal cells.

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