Nrp1 bv421

Manufactured by BioLegend

The NRP1·BV421 is a fluorochrome-conjugated antibody that specifically binds to the Neuropilin-1 (NRP1) receptor. NRP1 is a cell surface glycoprotein involved in various cellular processes. The BV421 fluorochrome allows for detection and analysis of NRP1-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Fc receptor block, by BD (1 mentions) Cd25 pe cy7, by BD (1 mentions) Cd4 apc cy7, by BD (1 mentions) Ki67 fitc, by Thermo Fisher Scientific (1 mentions) Intracellular fixation and permeabilization buffer set, by Thermo Fisher Scientific (1 mentions) Ctla 4 pe, by BD (1 mentions) Foxp3 apc, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Facsdiva, by BD (1 mentions) Facs lsrii flow cytometer, by BD (1 mentions) Cd152 apc, by BioLegend (1 mentions) Helios percp cy5, by BioLegend (1 mentions) Gitr pecy7, by BioLegend (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Cd44 bv510, by BioLegend (1 mentions) Cd62l fitc, by BioLegend (1 mentions) Tigit bv421, by BioLegend (1 mentions) Cd25 apc, by BioLegend (1 mentions)

2 protocols using nrp1 bv421

Immune Cell Analysis in Murine Lymph Nodes

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Single-cell suspensions were prepared from the dLN of Tlr4^+/+ and Tlr4^−/− females in estrus or on d 3.5 pc, incubated with Fc receptor block (BD Biosciences), and labeled with fluorophore-conjugated monoclonal antibodies against CD4·APC-Cy7, CD25·PE-Cy7 (both BD Biosciences), NRP1·BV421 (Biolegend, San Diego, CA), then treated with Intracellular Fixation and Permeabilization Buffer Set (Thermo Fisher Scientific) and labeled with antibodies against Ki67·FITC, FOXP3·APC (both Thermo Fisher Scientific), and CTLA4·PE (BD Biosciences), and analyzed on a FACS Canto II flow cytometer with FACSDiva (BD Biosciences) and FlowJo software (BD Biosciences) as described^{6 (link)}, using gating as shown in Supplemental Fig. S3. Count beads (CountBright™ Count Beads, Thermo Fisher Scientific) were used to calculate the total number of each cell type in each tissue, as described^{54 (link)}. In all samples, gates were established using unlabeled, fluorescence minus one and isotype-matched negative controls.

Chan H.Y., Moldenhauer L.M., Groome H.M., Schjenken J.E, & Robertson S.A. (2021). Toll-like receptor-4 null mutation causes fetal loss and fetal growth restriction associated with impaired maternal immune tolerance in mice. Scientific Reports, 11, 16569.

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Characterization of Lymph Node Immune Cells

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Ipsilateral draining submandibular and cervical lymph nodes were extracted from recipient mice (n = 6) of each group at the time of euthanization. The single-cell suspension from the lymph nodes was centrifuged and the final cell concentration was adjusted to 1 × 10 7 /mL. The 100-µl cell suspension was taken to the bottom of the flow tube. Surface staining was performed before intracellular staining. CD4-APC/C7 (Biolegend, clone # GK1.5), CD44-BV510 (Biolegend, clone # IM7), CD62-FITC (Biolegend, clone #MEL-14), CD152-APC (Biolegend, clone #UC10-4B9), GITR-PE/Cy7 (Biolegend, clone #DTA-1), Nrp-1-BV421 (Biolegend, clone #3E12), CD25-APC (Biolegend, clone #PC61), CD62L-FITC (Biolegend, clone #MEL-14), TIGIT-BV421 (Biolegend, clone #1G9), CD226-PE/Cy7 (Biolegend, clone #10E5), Helios-PerCP/Cy5.5 (Biolegend, clone #22F6), and Foxp3-PE (Thermo, clone# FJK-16s) were used in this study. Fluorescence-activated cell sorting (FACS) was performed using BD FACS LSRII Flow Cytometer. Fifty thousand lymphocytes for each sample were collected by FACS Diva 8.0 software and finally analyzed by Flowjo software (BD Bioscience, USA).

Li S., Zhang P., Li A., Bao J., Pan Z, & Jie Y. (2023). TIGIT-Fc Prolongs Corneal Allograft Survival in Mice by Upregulating TIGIT/CD226 Expression and the Proportion of Helios + Foxp3 + Treg Cells. Transplantation, 107(2).

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