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G6pdh antibody

Manufactured by Merck Group

The G6PDH antibody is a laboratory reagent used in various biochemical and diagnostic assays. It is an antibody that specifically binds to the glucose-6-phosphate dehydrogenase (G6PDH) enzyme, which plays a crucial role in the pentose phosphate pathway and is involved in cellular redox homeostasis. The G6PDH antibody can be used to detect and quantify the presence of the G6PDH enzyme in biological samples, but its specific applications and intended uses are not provided in this factual description.

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2 protocols using g6pdh antibody

1

Yeast Protein Extraction and Detection

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Whole cell extracts of yeast were made using the trichloroacetic acid (TCA) extraction method described in (Hager et al., 2018) , as modified from (Volland et al., 1994) .
Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase, referred to as G6PDH) antibody (Sigma, St. Louis, MO) as a protein loading control. Anti-mouse or anti-rabbit secondary antibodies conjugated to IRDye-800 or IRDye-680 (Li-Cor Biosciences, Lincoln, NE) were detected on an Odyssey TM FC infrared imaging systems (Li-Cor Biosciences).
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2

Yeast Protein Extraction and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell extracts of yeast were made using the trichloroacetic acid (TCA) extraction method described in (Hager et al., 2018) , as modified from (Volland et al., 1994) .
Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase, referred to as G6PDH) antibody (Sigma, St. Louis, MO) as a protein loading control. Anti-mouse or anti-rabbit secondary antibodies conjugated to IRDye-800 or IRDye-680 (Li-Cor Biosciences, Lincoln, NE) were detected on an Odyssey TM FC infrared imaging systems (Li-Cor Biosciences).
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