Rapamycin rapa

Manufactured by LC Laboratories

Sourced in United States

Rapamycin (Rapa) is a macrolide compound produced by the bacterium Streptomyces hygroscopicus. It functions as an immunosuppressant and anti-proliferative agent.

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Lab products found in correlation

Carbachol, by Merck Group (1 mentions) Nhs rhodamine, by Thermo Fisher Scientific (1 mentions) Sulfo cyanine 7.5 nhs ester, by Lumiprobe (1 mentions) Hb ap2100001, by Hanbio Biotechnology (1 mentions) Anti lc3 clone 2g6, by Nanotools (1 mentions) Anti gm130, by BD (1 mentions) Anti β actin mab, by Merck Group (1 mentions) Nucleoporin p62, by BD (1 mentions) Hsc70, by Thermo Fisher Scientific (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Anti cd9, by Abcam (1 mentions)

4 protocols using rapamycin rapa

Evaluating Ocular Tear Production

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BLR(DE3) competent E. coli was from New England BioLabs Inc. (Ipswich, MA, USA). Rapamycin (Rapa) was from LC Laboratories (Woburn, MA, USA). TO-PRO^™-3 Iodide (642/661) and NHS-Rhodamine were from ThermoFisher Scientific Inc. (Rockford, IL,USA). Carbachol, used to stimulate tear production, was from Sigma-Aldrich (St. Louis, MO, USA). Ketamine (Ketaject) was from Phoenix (St. Joseph, MO, USA) and xylazine (AnaSed) was from Akorn (Lake Forest, IL, USA). Zeba^™ Spin Desalting Columns, 7K MWCO (10 mL) were from ThermoFisher Scientific Inc. (Rockford, IL, USA). Sulfo-Cyanine 7.5 NHS ester was from Lumiprobe Corp (Hallandale Beach, FL, USA). ZoneQuick phenol red threads were from SHOWA YAKUHIN KAKO CO., LTD (Tokyo, Japan). Free Style Lite test strips were from Abbott Diabetes Care, Inc. (Alameda, CA, USA). Other reagents were from standard suppliers.

Ju Y., Edman M.C., Guo H., Janga S.R., Peddi S., Louie S.G., Junge J.A., MacKay J.A, & Hamm-Alvarez S.F. (2020). Intra-lacrimal Sustained Delivery of Rapamycin Shows Therapeutic Effects Without Systemic Toxicity in a Mouse Model of Autoimmune Dacryoadenitis Characteristic of Sjögren’s Syndrome. Biomacromolecules, 22(3), 1102-1114.

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Encapsulating Peptides in PLGA-PVA Nanoparticles

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Myelin oligodendrocyte glycoprotein (MOG_35–55, MOG) and ovalbumin (OVA_323–339, OVA) peptides, with or without a fluorescein isothiocyanate (FITC) tag conjugated to the N-terminus, were synthesized by GenScript at ≥ 98% purity. Poly(lactide-co-galactide) (PLGA) and poly(vinyl alcohol) (PVA) were purchased from Sigma. Rapamycin (Rapa) was purchased from LC Laboratories. Antibodies for flow cytometry, including CD16/CD32, V450 CD4 (clone RM4–5), PE-Cy7 CD25 (clone PC61), Alexa Fluor 488 Foxp3 (clone MF23), PE-Cy7 CD3 (clone 145-2C11), FITC CD4 (clone RM4–5), PE CD8 (clone 53–6.7), PE F4/80 (clone T45–2342) and allophycocyanin-Cy7 CD11c (clone HL3), were purchased from BD Biosciences.

Tostanoski L.H., Chiu Y.C., Gammon J.M., Simon T., Andorko J.I., Bromberg J.S, & Jewell C.M. (2016). Reprogramming the local lymph node microenvironment promotes tolerance that is systemic and antigen-specific. Cell reports, 16(11), 2940-2952.

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Macrophage Polarization and Autophagy

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For macrophage polarization detection, BMDMs from both WT and Nrf2^−/− mice were divided into control group and LPS-treated group. In each group, BMDMs were incubated with DMSO or LPS (1 μg/mL) for 6 h. The cells were then collected for flow cytometry analyses or immunofluorescence staining. For autophagy activation or inhibition, 0.1 μM Rapamycin (RAPA, LC Laboratories, Woburn, MA, USA, AY22989) or 2 mM 3-MA (Sigma-Aldrich, Saint Louis, MO, USA, M9281) were added in the culture media with the macrophage polarization inducers simultaneously. After 24 h of culture, cells were collected for subsequent analyses. The recombinant mRFP-GFP-LC3B lentivirus vector was purchased from Hanbio Biotechnology (Shanghai, China, HB-AP2100001). Cells were transfected with mRFP-GFP-LC3B plasmid after drug administration and harvested for autophagy analyses 24 h later.

Luo J., Wang J., Zhang J., Sang A., Ye X., Cheng Z, & Li X. (2022). Nrf2 Deficiency Exacerbated CLP-Induced Pulmonary Injury and Inflammation through Autophagy- and NF-κB/PPARγ-Mediated Macrophage Polarization. Cells, 11(23), 3927.

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Subcellular Protein Profiling Protocol

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All the reagents and chemicals were obtained from Sigma–Aldrich, unless otherwise indicated. Lipo-polysaccharide (LPS) (L2800), chloroquine diphosphate (CQ) (C6628) & Rapamycin (RAPA) was obtained from LC laboratories (R-5000). Sources of the antibodies were as follows: HSC70 (Thermo Scientific, PA5-27,337), anti-CD9 (Abcam, ab92726), Bcl2 (Cell Signaling, D17C4), nucleoporin P62 (BD Transduction Laboratories, 610,497), anti-GM130 (BD Transduction Laboratories, 610,822), anti-β-actin mAb (Sigma–Aldrich, A5441), anti-LC3 (clone 2G6) (NanoTools, 0260-100).

Atya H.B., Sharaf N.M., Abdelghany R.M., El-Helaly S.N, & Taha H. (2024). Autophagy and exosomes; inter-connected maestros in Alzheimer’s disease. Inflammopharmacology, 32(3), 2061-2073.

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