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1 2 dioleoyl sn glycero 3 phosphocholine

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1,2-dioleoyl-sn-glycero-3-phosphocholine is a synthetic lipid compound. It is a phospholipid that consists of two oleic acid chains attached to a glycerol backbone, with a phosphocholine headgroup.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (55 mentions) 1 2 dioleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (36 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (34 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (31 mentions) 1 2 dioleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (15 mentions) Chloroform, by Merck Group (15 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (14 mentions) 1 2 dioleoyl sn glycero 3 phosphoglycerol, by Avanti Polar Lipids (10 mentions) 1 2 diphytanoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (9 mentions) Sucrose, by Merck Group (9 mentions) Sodium chloride (nacl), by Merck Group (8 mentions) 1 2 dilauroyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (8 mentions) 1 2 dioleoyl sn glycero 3 phospho l serine sodium salt, by Avanti Polar Lipids (7 mentions) L α phosphatidylinositol 4 5 bisphosphate, by Avanti Polar Lipids (7 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (7 mentions) Cholesterol, by Merck Group (7 mentions) Triton x 100, by Merck Group (7 mentions) 1 2 distearoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (7 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (6 mentions) Bovine serum albumin (bsa), by Merck Group (6 mentions)

264 protocols using 1 2 dioleoyl sn glycero 3 phosphocholine

1

Functionalized PEG-based Hydrogel Synthesis

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Thiol end-functionalized four-arm PEG (5 kDa, PEG-SH4) and vinylsulfone end-functionalized four-arm PEG (5 kDa, PEG-VS4) were purchased from JenKem Technology USA Inc. (Allen, TX). Fluorescent FITC-dextran (4 kDa) was purchased from Sigma-Aldrich (St. Louis, MO). All lipids were purchased from Avanti Polar Lipids (Alabaster, AL), including: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), 1,2-dioleoylsn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide] (MPB-PE), and 1,2-dioleoyl-sn-glycero-3-phosphocholine-N-(Cyanine 5) (Cy5-PC).
Palmese L.L., LeValley P.J., Pradhan L., Parsons A.L., Oakey J.S., Abraham M., D’Addio S.M., Kloxin A.M., Liang Y, & Kiick K.L. (2024). Injectable liposome-containing click hydrogel microparticles for release of macromolecular cargos. Soft Matter, 20(8), 1736-1745.
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2

Nanoparticle Synthesis and Characterization

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PLGA (75:25 L:G; ester-terminated, inherent viscosity range: 0.55–0.75 dL/g in CHCl3) was purchased from Lactel. All lipids for the nanoparticle synthesize were purchased from Avanti Polar Lipids, including 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG-MAL). Cholesteryl butyrate was purchased from Santa Cruz Biotechnology. Rhesus recombinant anti-CD4 antibody and rhesus recombinant IgG1 isotype control antibody were purchased from NIH Nonhuman Primate Reagent Resource. FITC mouse anti-human CD8, PE mouse anti-human CD14, PerCP mouse anti-human CD3 antibodies, FITC mouse anti-human CD4, and FITC mouse IgG1, PE mouse IgG2a, PerCP mouse IgG1 κ control antibodies were purchased from BD Biosciences. RPMI 1640 containing 2 mMl-glutamine and 25 mM HEPES, DPBS, heat-inactivated fetal bovine serum (FBS), Penicillin–Streptomycin (10,000 U/mL), 1,1′ -Dioctadecyl-3,3,3′, 3′ -Tetramethylin-dodicarbocyanine, 4-Chlorobenzenesulfonate Salt (DiD), LIVE/DEAD® Fixable Violet Dead Cell Stain Kit, Dylight 633 NHS Ester were purchased from ThermoFisher. All other chemicals were purchased from Sigma-Aldrich and Fisher Scientific unless otherwise specified.
Cao S., Jiang Y., Levy C.N., Hughes S.M., Zhang H., Hladik F, & Woodrow K.A. (2018). Optimization and comparison of CD4-targeting lipid–polymer hybrid nanoparticles using different binding ligands. Journal of biomedical materials research. Part A, 106(5), 1177-1188.
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3

Lipid-based Delivery of PNA-a210

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Egg Phosphatidylcholine (Egg PC) 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2dioleoyl-sn-glycero-3-phosphocholine (DOPG), L-α-phosphatidylcholine hydrogenated soy (HSPC), cholesterol (CHO) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carbonylmethoxy(polyethylene glycol)2000] (ammonium salt) (DSPE-PEG2000) were purchased from Avanti Polar Lipids (Alabaster, AL). Dialysis tubing 8-10 kDa MWCO was purchased by Pierce Protein (USA). The PNA-a210 was prepared as described elsewhere (18) . The sequence of PNA-a210 follows: H-ccgctgtcacacgcacag-NH 2 .
Ringhieri P., Avitabile C., Saviano M., Morelli G., Romanelli A, & Accardo A. (2016). The influence of liposomal formulation on the incorporation and retention of PNA oligomers. Colloids and surfaces. B, Biointerfaces, 145.
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4

Lipid Membrane Composition Analysis

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Materials. Deuterium oxide (D2O, 99.9%), DMSO-d6 (99.9%), calcein, sodium chloride (NaCl, >99.5%), urea, tris(hydroxymethyl)aminomethane (tris), sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich. Phosphate-buffered saline (PBS) was from Vivantis Technologies (Selangor, Malaysia). 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), egg sphingomyelin (SM), cholesterol, and lissamine rhodamine B 1,2-dipalmitoeyl-sn-glycero-3-phosphoethanolamine (Rhod-DPPE) were purchased form Avanti Polar Lipid (Alabaster, Alabama, USA). 4BAH2 (H-WRTLEAFWAKHMWNFISGIQYLA-NH2) were acquired from Anaspec Inc. (Fremont, CA). All chemicals were used without further purification. 96-well plates were obtained from Ibidi GmbH (Planegg / Martinsried, Germany).
Ho J.C.S., Steininger C., Hiew S.H., Kim M.C., Reimhult E., Miserez A., Cho N., Parikh A.N, & Liedberg B. (2019). Minimal Reconstitution of Membranous Web Induced by a Vesicle-Peptide Sol-Gel Transition. Biomacromolecules, 20(4).
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5

Lipid-based Nanomedicine Formulation

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2-HydroxyOleic acid (sodium salt, 2OHOA), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were purchased from Avanti Polar Lipid Inc. (Alabaster, AL). Oleic acid and ATRA were purchased from Sigma-Aldrich Inc. (St. Louis, MO). Mitoxantrone dihydrochloride was bought from Tocris Bioscience Inc. (Bristol, UK). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Amresco (Solon, OH). Paclitaxel was purchased from LC Laboratories (Boston, MA). All other chemicals were of reagent grade and used without further purification.
Jang E.J., Choi W.R., Kim S.Y., Hong S.S., Rhee I., Lee S.J., Choi S.W., Choi H.G, & Lim S.J. (2017). 2-Hydroxyoleic acid-inserted liposomes as a multifunctional carrier of anticancer drugs. Drug Delivery, 24(1), 1587-1597.
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6

Amyloid Peptide and Lipid Interaction

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Rat-amylin (rIAPP) and amyloid-β 1–40 (Aβ1‑40) were purchased from Bachem (Bubendorf, Switzerland) with a purity >98%. 1,2-Dierucoyl-sn-glycero-3-phosphocholine (PC 22:1), 1,2-dieicosenoyl-sn-glycero-3-phosphocholine (PC 20:1), 1,2-dioleoyl-sn-glycero-3-phosphocholine (PC 18:1), 1,2-dipalmitoleoyl-sn-glycero-3-phosphocholine (PC 16:1), and 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine (PC 14:1) were purchased by Avanti Polar Lipid (Alabaster, AL, USA); 6-carboxyfluorescein, Fura-2, Thioflavin T (ThT), 1,1,1,3,3,3-hexa-fluoro-2-propanol (HFIP), and all other salts were purchased from Sigma-Aldrich (St.Louis, MO) with a purity of 99%.
Sciacca M.F., Lolicato F., Tempra C., Scollo F., Sahoo B.R., Watson M.D., García-Viñuales S., Milardi D., Raudino A., Lee J.C., Ramamoorthy A, & La Rosa C. (2020). Lipid-Chaperone Hypothesis: A Common Molecular Mechanism of Membrane Disruption by Intrinsically Disordered Proteins. ACS chemical neuroscience, 11(24), 4336-4350.
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7

Purification and Production of KRAS4b

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Fully processed KRAS4b was produced and purified as described before (85 (link)). All lipids were purchased from Avanti Polar Lipids (Alabaster): 1,2-dioleoyl-sn-glycero-3 phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine sodium salt (DOPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine sodium salt (POPS).
Liang H., Mu H., Jean-Francois F., Lakshman B., Sarkar-Banerjee S., Zhuang Y., Zeng Y., Gao W., Zaske A.M., Nissley D.V., Gorfe A.A., Zhao W, & Zhou Y. (2019). Membrane curvature sensing of the lipid-anchored K-Ras small GTPase. Life Science Alliance, 2(4), e201900343.
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8

Purification of Coagulation Factors

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Protein S-free C4BP was purified (29) and APC was activated from in house purified protein C as described (30) (31) (32) . Recombinant FV R506Q/R679Q was expressed and quantified as described (33) , bovine FX (34) and human prothrombin were purified from plasma (35) .
Human α-thrombin was prepared from purified prothrombin (36) . Phospholipids were purchased from Avanti Polar Lipids and include the natural phospholipids phosphatidylserine (PS; brain extract), phosphatidylethanolamine (PE; egg extract) and phosphatidylcholine (PC; egg extract) and the synthetic phospholipids 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-Dioleoyl-sn-glycero-3-phosphoserine (DOPS), and 1,2-Dioleoyl-sn-glycero-3phosphoethanolamine (DOPE). Phospholipid vesicles were prepared by extrusion technique (37) .
Somajo S., Ahnström J., Fernandez-Recio J., Gierula M., Villoutreix B.O, & Dahlbäck B. (2015). Amino acid residues in the laminin G domains of protein S involved in tissue factor pathway inhibitor interaction. Thrombosis and haemostasis, 113(5).
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9

Comprehensive Coagulation Cascade Assay

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Full-length (residues 1-263) recombinant tissue factor (TF) and factor (F) XIII were purchased from Haematologic Technologies (Essex Junction, VT, USA). FXIIa was purchased from Enzyme Research Laboratories (South Bend, IN, USA). 1,2-Dioleolyl-sn-Glycero-3-Phospho-L-Serine (PS) and 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (PC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Phospholipid vesicles (PCPS) composed of 75% PC and 25% PS were prepared as described [22] , as were the TF/PCPS reagent and corn trypsin inhibitor (CTI) [23] . Antithrombin (AT) and factors II, V, VII, IX, X and XI were isolated from freshly frozen plasma [24] . Fibrinogen was prepared as described [25] . Recombinant human FVIIa was provided as a gift by Dr Lars Pedersen (Novo Nordisk, Bagsvaerd, Denmark). Recombinant full-length tissue factor pathway inhibitor-a (TFPI) was a gift from Dr Johnson (Chiron, Emeryville, CA, USA). Recombinant FVIII was a gift from Dr Lundblad (Baxter Healthcare, Duarte, CA, USA). D-Phe-Pro-Arg-CH 2 Cl (FPR-ck) [26] and a-thrombin [27] were produced in-house.
Gissel M., Brummel-Ziedins K.E., Butenas S., Pusateri A.E., Mann K.G, & Orfeo T. (2016). Effects of an acidic environment on coagulation dynamics. Journal of thrombosis and haemostasis : JTH, 14(10).
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10

Lipid Bilayer Formation and Characterization

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The lipids used in the study were 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), purchased from Avanti Polar Lipids; porcine globotriaosylceramide (Gb 3 ) was purchased from Matreya LLC. The Shiga toxin B unit (STxB) was supplied by Prof. Ludger Johannes, Institut Curie, UMR144 Curie/ CNRS, Paris, France. 8 All lipids were dissolved in methanol before sample preparation. All used chemicals were of HPLC grade quality and Milli-Q water was used for the buffer preparation. HEPES buffer (5 mM, 150 mM NaCl), pH7.0 was prepared by mixing HEPES acid, HEPES base and NaCl components. Muscovite mica from Plano GmbH (Germany) was cut into approx. 10 Â 10 mm 2 pieces, freshly cleaved, and used as a substrate.
Solovyeva V., Johannes L, & Simonsen A.C. (2015). Shiga toxin induces membrane reorganization and formation of long range lipid order. Soft matter, 11(1).
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