BMSCs (ATCC)were cultured in MEMα medium supplemented with 10% fetal bovine serum (FBS; Gibco), 1% antibiotic‐anti‐mycotic solution (Gibco) and 5 ng/ml bFGF (Gibco). Osteogenic differentiation media comprised the basal culture medium supplemented with 50 μM L‐ascorbic acid (Sigma), 10 mM β‐sodium glycerophosphate (Sigma) and 10 nM dexamethasone (Sigma). MC3T3 cells (Chinese Academy of Medical Sciences, China) were cultured in MEMα medium supplemented with 10% FBS (Gibco), 1% antibiotic‐anti‐mycotic solution (Gibco). RAW264.7 cells (RAW‐OCs, Chinese Academy of Medical Sciences)were cultured in DMEM medium supplemented with 10% FBS (Gibco) and 1% antibiotic‐anti‐mycotic solution (Gibco). The generation of colonic organoids from hiPSCs (CELLAPYBIO) involved a multistep technique whereby hiPSCs were directed to form definitive endoderm, hindgut structures and ultimate colonic organoids. hiPSCs were grown on matrigel (BD)‐coated six‐well plates in mTsSR1 medium (STEM CELL Technologies). All cells were maintained at 37°C with 5% CO2.
Bmscs
Manufactured by American Type Culture Collection
Sourced in United States, China
BMSCs are a type of cell culture that is derived from bone marrow. They are multipotent stromal cells that have the ability to differentiate into various cell types, including osteoblasts, chondrocytes, myocytes, and adipocytes. BMSCs are commonly used in research and clinical applications related to tissue regeneration and repair.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (11 mentions)
Penicillin, by Thermo Fisher Scientific (5 mentions)
Streptomycin, by Thermo Fisher Scientific (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Bmscs, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Corning (2 mentions)
Streptomycin, by Corning (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Huvecs, by American Type Culture Collection (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions)
Mtssr1 medium, by STEMCELL (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
Sodium β glycerophosphate, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Sismad3, by GenePharma (1 mentions)
26 protocols using bmscs
1
Diverse Cell Culture Protocols for Tissue Engineering
2
Effect of Dexamethasone on miR-596 Expression in BMSCs
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BMSCs (ATCC, Manassas, VA, USA) were maintained in a complete medium containing with 100 U/ml penicillin, 10% fetal bovine serum, and 100 μg/ml streptomycin (all from Gibco; Waltham, USA) and cultured at 37 °C and 5% CO2. Different concentrations (10-8 M, 10-7 M, and 10-6 M) of GC—dexamethasone (Dex), was added into the medium, and miR-596 expression was measured by quantitative real-time PCR (qRT-PCR). The cell morphology of BMSCs n the first and fourteenth day after GC induction was observed under an inverted microscope. MiR-596 mimics, miR-596 inhibitor, si-Smad3, and their negative control (NC) were procured from Shanghai GenePharma, Co., Ltd. (China). Following the manufacturer’s protocol, cell transfections were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, USA).
3
Sterilization and Osteogenesis of BMSCs
4
Establishing In Vitro Bone-Prostate Cancer Co-Culture Model
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The human PCa cell lines PC-3 derived from metastatic bone site, mouse preosteoblastic cell line MC3T3-E1, and human osteoblast cells (hFOB1.19) were obtained from the Shanghai Chinese Academy of Sciences cell bank (Shanghai, China). PC-3 was cultured in RPMI-1640 medium (c11875500bt; GIBCO) supplemented with 10% FBS (10099-141; GIBCO), MC3T3-E1 in Alpha Minimum Essential Medium (A1049001; GIBCO), and hFOB1.19 in F-12 medium (12400024; GIBCO). The LNCaP-derived bone metastatic cell line C4-2B was purchased from MD Anderson Cancer Center and maintained in T-medium (A1048501; GIBCO) supplemented with 10% FBS (Wu et al., 1998 (link)). BMSCs were purchased from ATCC and cultured according to the ATCC protocol. All cell lines were grown under a humidified atmosphere of 5% CO2 at 37°C, except for hFOB1.19 at 33.5°C. For the transwell co-culture model, different densities of PCa cells were seeded on the lower chamber of 6-well plate according to the requirements of different assays, and the culture insert with 0.4-µm pore size (3412; Corning) was placed on the top of each well followed by seeding with 5 × 105 osteoblasts in the upper chamber of transwell.
5
Culturing Hepatoblastoma and Liver Cells
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Hepatoblastoma cell lines (huH6 and HepG2), normal liver cells (WRL68), and human bone marrow mesenchymal stem cells (BMSCs) were bought from ATCC (Manassas, VA, USA). Hepatoblastoma cells and WRL68 cells were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% FBS and 1% streptomycin and penicillin (Thermo Fisher Scientific) in an incubator (37°C, 5% CO2). BMSCs were maintained in α-MEM (Gibco) with 10% FBS (Corning) and 1% streptomycin and penicillin. The cells were maintained under the following conditions: 5% CO2 and 95% humidity.
6
Cultivation of Multiple Myeloma Cell Lines
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Human MM cells MM.1R, MM. 1S, U266, RPMI 8226 cells, and BM stromal cells (BMSCs) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). Bortezomib resistant cells MM.1S BTZ-R and RPMI8226 BTZ-R were a generous gift from Dr. Nathan Dolloff, The Medical University of South Carolina, Charleston, SC, USA. MM.1R, MM. 1S, and RPMI 8226 cells were cultured in RPMI 1640 growth media (Cellgro, Manassas, VA) supplemented with heat-inactivated 10% Fetal Bovine Serum (Sigma, St. Louis, MO), 100 I.U./ml penicillin, and 100 μg/ml streptomycin (Cellgro, Manassas, VA). U266 cells were cultured in RPMI 1640 with 15% FBS. BMSCs were procured from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (ATCC) supplemented with heat-inactivated 10% Fetal Bovine Serum (Sigma), 100 I.U./ml penicillin, and 100 μg/ml streptomycin (Cellgro, Manassas, VA). All cells were cultured in a 37°C and 5% CO2 incubator.
7
Sterilization and Osteogenesis of BMSCs
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The MEFs and control samples were sterilized by immersion in 70% ethanol for 10 min, and then all sides of the samples were exposed to ultraviolet light for 15 min. Then, the samples were washed with sterilized phosphate buffer solution. Mouse bone marrow-derived mesenchymal stem cells (BMSCs, CRL-12424, ATCC, Manassas, VA, USA), which were tested to be free from mycoplasma contamination, were cultured until achieving ~ 75% confluence, and the BMSCs, before passage 5, were used for an all cell assay. BMSCs were trypsinized and seeded in 24-well plates in normal growth media (10% fetal bovine serum+Dulbecco’s modified Eagle’s medium, Gibco) at a density of 5000 cells per cm2 for morphology observation and at 10 000 cells per cm2 for the osteogenesis experiments. The samples with the seeded cells were placed in a 37 °C incubator under a 5% CO2 atmosphere.
8
Mouse Osteogenic Cell Culture and BMSC Isolation
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Mouse osteogenic cell line MC3T3-E1, purchased from American Type Culture Collection (ATCC), was cultured with DMEM medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. MC3T3-E1 cells were suitable for growth in a humidity adjustable environment containing 5% CO2 at 37°C. Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO2 at 37°C.
9
Rat BMSC and HUVEC Co-culture Protocol
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Rat bone marrow–derived mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) were obtained from ATCC (USA) and their culture conditions were previously described [30 ]. Phosphate-buffered saline (PBS), Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and live/dead viability kit were purchased from Thermo Fisher Scientific. Needles were purchased from BD Biosciences (USA). TA (Selleck, USA) was dissolved in dimethyl sulfoxide (DMSO; Sigma–Aldrich, Shanghai, China) and stored at −20 °C. GelMA, gelatin, and PCL were purchased from SunP Biotech (Beijing, China). Primary antibodies of anti-COL-I, COL-II, Runx2, OCN, VEGF, CAV-1, β-catenin, GSK-3β, and p-GSK-3β were purchased from Abcam (Cambridge, UK). gelatin (A type, derived from porcine skin), photoinitiator (PI, purity: 98%), alcian blue, and Alizarin Red S (ARS) were purchased from Sigma–Aldrich. Methacrylic anhydride (MA) was supplied by Shanghai Macklin Bio-Chem Technology Co., Ltd. (China). nHA was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (China).
10
Culturing BMSCs from ATCC
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BMSCs were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in the same manner as the ADSCs and UCSCs, as described above.
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