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The SCC-9 is a laboratory instrument designed for the culture and maintenance of cells. It provides a controlled environment for the growth and propagation of various cell lines. The SCC-9 features temperature, humidity, and atmospheric gas regulation to support optimal cell culture conditions.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Cal27, by National Collection of Authenticated Cell Cultures (7 mentions) Tca8113, by National Collection of Authenticated Cell Cultures (6 mentions) Tscca, by National Collection of Authenticated Cell Cultures (5 mentions) Scc25, by National Collection of Authenticated Cell Cultures (4 mentions) Sh hoxa as3, by Genechem (3 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (3 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Sangon (1 mentions) Mir 218 5p mimics, by RiboBio (1 mentions) Abi 7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Genome extraction kit, by Corning (1 mentions) Scrambled oligonucleotides nc, by GenePharma (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Pcdna3, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Dmem f12 1 1 medium, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Cal27, by American Type Culture Collection (1 mentions) Hek293t, by National Collection of Authenticated Cell Cultures (1 mentions)

13 protocols using scc 9

1

Oral Squamous Cell Carcinoma Cell Line Cultivation and Transfection

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Four OSCC cell lines (TSCCA, CAL-27, SCC-9, and Tca8113) and the normal human oral keratinocyte (NHOK) were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, PRC). All cells were cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA), 100 IU/mL of penicillin and 100 μg/mL of streptomycin at 37 °C incubator with 5% CO 2 .
shRNA containing the HOXA-AS3 interference sequence (sh-HOXA-AS3) and negative control (sh-NC) were purchased from GeneChem (Shanghai, PRC), and miR-218-5p mimics, anti-miR-218-5p and negative control (miR-NC) were purchased from RiboBio (Guangzhou, PRC). Transfection was performed using Lipofectamine® 2000 Reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer's protocol in OSCC cells. The culture medium was replaced 6 h after transfection, and transfection e ciency was examined with the expression vector of red uorescent protein (RFP) at 48 h after the transfection.
Zhao Y., & Yao R. (2020). LncRNA HOXA-AS3 promotes the progression of oral squamous cell carcinoma through sponging miR-218-5p.
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2

Generating CDDP-Resistant Oral Cancer Cells

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The normal human oral keratinocyte (NHOK) and OSCC cell lines, including TSCCA, CAL-27, SCC-9, and Tca8113, were ordered from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). TSCCA and CAL-27 cells were exposed to gradually increasing doses of CDDP to establish CDDP-resistant OSCC cells (TSCCA-CDDP and CAL-27-CDDP). All cells were maintained in DMEM medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin in a humidified atmosphere of 5% CO2.
Wang X., Li H, & Shi J. (2019). LncRNA HOXA11-AS Promotes Proliferation and Cisplatin Resistance of Oral Squamous Cell Carcinoma by Suppression of miR-214-3p Expression. BioMed Research International, 2019, 8645153.
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3

OSCC Cell Lines Cultivation and Transfection

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Four OSCC cell lines (TSCCA, CAL-27, SCC-9, and Tca8113) and the normal human oral keratinocyte (NHOK) were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, PRC). All cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA), 100 IU/mL of penicillin and 100 μg/mL of streptomycin at 37 °C incubator with 5% CO2. shRNA containing the HOXA-AS3 interference sequence (sh-HOXA-AS3) and negative control (sh-NC) were purchased from GeneChem (Shanghai, PRC), and miR-218-5p mimics, anti-miR-218-5p and negative control (miR-NC) were purchased from RiboBio (Guangzhou, PRC). Transfection was performed using Lipofectamine® 2000 Reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer's protocol in OSCC cells. The culture medium was replaced 6 h after transfection, and transfection efficiency was examined with the expression vector of red fluorescent protein (RFP) at 48 h after the transfection.
Zhao Y., & Yao R. (2020). LncRNA HOXA-AS3 promotes the progression of oral squamous cell carcinoma via inhibiting miR-218-5p.
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4

Culturing OSCC Cell Lines

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Normal human oral keratinocyte (NHOK), DDP-resistant OSCC cells (SCC-9/DDP and CAL-27/DDP) and OSCC cell lines (CAL-27, Tca8113, SCC-9, TSCCA) were all bought from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China).
All these cells were cultured in DMEM (Gibco, Rockville, MD, USA) containing 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA) at 37°C in an incubator with 5% CO2.
Wang X., Hao R., Wang F, & Wang F. (2020). ZFAS1 Promotes Cisplatin Resistance via Suppressing miR-421 Expression in Oral Squamous Cell Carcinoma. Cancer Management and Research, 12, 7251-7262.
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5

Culturing Human Oral Cell Lines

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Human OSCC cell lines (Cal27, SCC9, and HSU3) and normal oral keratinocytes (HOK) were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Cells were cultured in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Sangong Biotech), and incubated at 37 ◦C in a humidified atmosphere of 5% CO2.
Zhang H.Y, & Ma J.H. (2020). miR-105 Promotes the Progression and Predicts the Prognosis for Oral Squamous Cell Carcinoma (OSCC). Cancer Management and Research, 12, 11491-11499.
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6

Culturing Oral Keratinocytes and OSCC Cell Lines

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Healthy human oral keratinocytes (HOKs) were bought from ScienCell Research Laboratories, Inc. (cat. no. 2610) and OSCC cell lines (CAL-27, HSC-4 and SCC-9) were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. Cells were cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin in a humidified incubator at 37˚C with 5% CO2.
Jin X., Huang T., Ma C., Duan J., Li R., Zhang W, & Tian W. (2021). Protein tyrosine kinase 7-knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression. Experimental and Therapeutic Medicine, 22(6), 1372.
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7

Characterization of Oral Squamous Cell Carcinoma

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The human tongue squamous carcinoma cells, SCC4 and SCC9, and the human normal skin broblast cell line, HF, were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences (Shanghai, China). The SCC4 and SCC9 cells were veri ed by The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences. No misidenti ed and/or contaminated cell lines were used in the present study.
The cells were 12th generations and cultured in the mixture medium, which contained Dulbecco's Modi ed Eagle's Medium (DMEM)/F12 (ThermoFisher Scienti c, Shanghai, China), 2 mM glutamine, 10% fetal bovine serum, 100 U/ml penicillin and 100 mg/ml streptomycin (ThermoFisher Scienti c, Shanghai, China). The culture conditions were 37˚C with a humidi ed atmosphere containing 5% CO 2 .
Zhou T., He Y., Dong X., Chen B., Ton J., Xiyang Y., & Mao R. (2021). MiR-219-5p is Involved in the Proliferation, Migration and Invasion of Oral Cancer Through SOX5 Regulation.
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8

HNSCC Cell Line Cultivation Protocol

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HNSCC cell lines, including HN-4, HN-9, HN-30, SCC-4, SCC-9, and SCC-25 cells, were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), and were cultured and stored according to the guidelines of the cell bank. The culture medium used was Dulbecco’s modified Eagle’s medium (DMEM; Winsent, Quebec, Canada) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 µg/ml streptomycin. All the cell lines were incubated in a 5% CO2 humidified incubator at 37°C.
Zhang S., Han J, & Fu J. (2021). The circ_0032822 Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma Cells Through miR-141/EF3 Signaling Axis. Frontiers in Oncology, 11, 662496.
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9

OSCC Cell Line Cultivation and Transfection

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Four OSCC cell lines (TSCCA, CAL-27, SCC-9, and Tca8113) and NHOK cells were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, PRC). All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Gibco, Waltham, MA, USA), 100 IU/mL of penicillin, and 100 μg/mL of streptomycin at 37°C incubator with 5% CO2.
shRNA containing the HOXA-AS3 interference sequence (sh-HOXA-AS3) and negative control (sh-NC) were purchased from GeneChem (Shanghai, PRC) and miR-218-5p mimics, anti-miR-218-5p and negative control (miR-NC) were purchased from RiboBio (Guangzhou, PRC). Transfection was performed using Lipofectamine® 2000 Reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer’s protocol in OSCC cells. The culture medium was replaced 6 h after transfection, and transfection efficiency was examined with the expression vector of red fluorescent protein (RFP) at 48 h after the transfection.
Zhao Y, & Yao R. (2021). Long non-coding RNA HOXA-AS3 promotes cell proliferation of oral squamous cell carcinoma through sponging microRNA miR-218-5p. Bioengineered, 12(1), 8724-8737.
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10

Verification of OSCC Cell Line Identity

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The OSCC cell lines including SCC-9, SCC-25, HN4, Tca-811 and hNOK cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human normal oral keratinocyte cell line hNOK cells were used as the negative control. For cell lines, we extracted DNA from various cells (SCC 9, SCC 25, HN4, Tca 811 and hNOK) using the Genome extraction kit of Axygen, amplified by the 21-STR amplification scheme. And then we detected STR spot and sex gene Amelogenin on the ABI 7900HT Fast Real-Time PCR System (Supplementary Table 2–6). No multiple alleles were found in these cell lines in this test. All cells were cultured in DMEM medium supplemented with 10% FBS and 100 μg/mL penicillin/streptomycin at 37°C with 5% CO2.
Zhang N., Zeng L., Wang S., Wang R., Yang R., Jin Z, & Tao H. (2021). LncRNA FER1L4 Promotes Oral Squamous Cell Carcinoma Progression via Targeting miR-133a-5p/Prx1 Axis. OncoTargets and therapy, 14, 795-806.
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