Liaison analyzer 310360

At the beginning and end of the study, ten ccs of venous blood samples were taken, and after the separation of serum, SIRT and Fetuin levels were measured in the plasma of the participants. Blood samples were collected into tubes with and without EDTA. Blood samples without EDTA Centrifuged (Beckman Avanti J-25, USA) at a rate of 3000 rpm for 10 min in order to the separation of serum. Blood samples were kept at − 70 °C (Snijdes, Germany) in Diabetes Research Center and then transferred to the laboratory of Velayat Hospital of Qazvin University of Medical Sciences, and the measurements were carried out. Fasting plasma glucose (FPG) concentration was measured by the enzymatic method using an Abbot ModelAclyon 300, USA auto analyzer with Pars-Azmone kit (Tehran, Iran). Plasma insulin was measured by using a chemiluminescent immunoassay method (LIAISON analyzer (310360) Diasorin S.P.A., Verecelli, Italy). Insulin resistance (HOMA-IR) was calculated according to the following formula: HOMA-IR = (fasting insulin (U/ml) × FPG (mg/dl)/405) [17 (link)]. Serum levels of SIRT1 and Fetuin-A in participants’ plasma were measured using a special kit and by ELISA method (Diameter, Italy and Bioassay Technology Laboratory, China).

At the beginning and end of the study, 10 cc of venous blood samples were taken and after separation of serum, biochemical parameters of glycemic status including fasting blood glucose (FBG), 2-hour Post Prandial (2hpp), (2-hour after breakfast), and HbA1c%, insulin and CRP measured in the plasma of the participants. Tubes with and without EDTA were used to collect blood samples. Tubes without EDTA were centrifuged (Beckman Avanti J-25, USA, 3000 rpm,10 min) and finally maintained at minus − 70 ° C for final measurements. FBG concentration was measured by the enzymatic method using an Abbot ModelAclyon 300, USA auto analyzer with Pars-Azmone kit (Tehran, Iran). The percentage of HbA1c was determined by high performance liquid chromatography (HPLC). Plasma insulin was measured by using a chemiluminescent immunoassay method (LIAISON analyzer (310,360) Diasorin S.P.A., Verecelli, Italy). Insulin resistance (IR) was calculated according to the following formula: Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) (µU/ml) = (Fasting insulin (U/ml) × FBG (mg/dl)/405) [18 (link)]. CRP concentration was measured by using an immune turbid metric assay (Pars Azmoon kit. Iran).