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Mgso4

Manufactured by Guangzhou Chemical
Sourced in China

MgSO4 is a laboratory chemical compound commonly used as a desiccant and drying agent. It is a crystalline solid with the chemical formula MgSO4. MgSO4 is soluble in water and has a high affinity for water molecules, making it effective in removing moisture from various solutions and environments.

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5 protocols using mgso4

1

Keratinocyte serum-free medium protocol

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Keratinocyte serum-free medium (K-SFM), Hank’s balanced salt solution, trypsin, penicillin, and streptomycin were purchased from Gibco (Carlsbad, CA, USA). Fetal bovine serum was purchased from Hyclone Laboratories (Logan, UT, USA). ACh, L-NG-nitro-arginine (L-NNA), indomethacin, prostaglandin E2 (PGE2), U73122, and thapsigargin (Tg) were purchased from Sigma Aldrich (St. Louis, MO, USA). Fluo-3 AM was purchased from Molecular Probes (Eugene, OR, USA). Atropine sulfate was purchased from Hengjian Pharmacy (Jiangmen, China). NaCl, KCl, CaCl2, MgCl2, NaHCO3, MgSO4, KH2PO4, Glucose, and Na2HPO4 were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). N-2-hydroethypiperazine-N’-2-ethanessulfonic acid (HEPES) was purchased from Biocolor BioScience & Technology (Shanghai, China).
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2

Inflammatory Signaling Pathway Modulation

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NaCl, KCl, MgSO 4 , NaHCO 3 , KH 2 PO 4 , glucose, and CaCl 2 were purchased from Guangzhou Chemical Pharmaceutical Factory (Guangzhou, China). Carbachol (CCh), genistein, GF109203X, PP2, nifedipine, and collagenase I were from Sigma Aldrich (St Louis, MO). Recombinant mouse TNF-α was purchased from R&D Systems (Minneapolis, MN) and was dissolved in Dulbecco's modified Eagle's medium (DMEM). DMEM and penicillin-streptomycin were purchased from Hyclone (Logan, UT). DMEM/F-12 and fetal bovine serum were purchased from Gibco (Grand Island, NY). Pronase, protease, and phosphatase inhibitor cocktail were purchased from Roche (Basel, Switzerland). Horseradish peroxidase substrate was purchased from Merck Millipore (Darmstadt, Germany).
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3

Reagents for Cell Culture Experiments

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Minimum essential medium (MEM), fetal bovine serum (FBS), penicillin/streptomycin, Hanks Balanced Salt Solution, sodium pyruvate and trypsin were purchased from Gibco (Carlsbad, CA, United States). 5-Alpha-dihydrotestosterone (5α-DHT), collagenase IA, pyridoxal 5-phosphate, O-(carboxymethyl) hydroxylamine hemihydrochloride chloride (AOAA), DL-propargylglycine (PAG), sodium hydrosulfide hydrate (NaHS × H2O), sodium lactate, L-cysteine (L-Cys), bumetanide, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), glibenclamide (Glib), Iberiotoxin (IbTx) and tetraethylammonium chloride (TEA) were purchased from Sigma-Aldrich (St. Louis, MO, United States). HC067047 was purchased from Tocris (Bristol, United Kingdom). NaCl, KCl, MgSO4, FeCl3, HCl, BaCl2, NaHCO3, KH2PO4, CaCl2, glucose, mannitol, lactic acid, normal saline, N,N-dimethyl-p-phenylenediamine sulfate, trichloroacetic acid, and zinc acetate were purchased from Guangzhou Chemical Pharmaceutical Factory (Guangzhou, China). Universal two-step detection kit (PV-9000) and DAB detection kit were purchased from ZSBIO (Beijing, China). Fluo-3 AM was purchased from Molecular Probes (Eugene, OR, United States). PBFI AM was purchased from Cayman Chemical (Ann Arbor, MI, United States).
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4

Allicin Modulates CFTR Channel Activity

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Minimum essential medium, fetal bovine serum, penicillin/streptomycin and trypsin were purchased from Gibco (Grand Island, NY, United States). Allicin was provided by Ailexin (Urumuqi, China). Amiloride, CFTRinh-172, DIDS, forskolin (FSK), isobutylmethylxanthine (IBMX) were purchased from Sigma-Aldrich (Missouri, United States). SQ22536 was purchased from MedChemExpress (New Jersey, United States). NaCl, KCl, MgSO4, NaHCO3, KH2PO4, CaCl2, glucose, and gluconate were purchased from Guangzhou Chemical Pharmaceutical Factory (Guangzhou, China). HEPES was purchased from Mbchem Technology (Guangzhou, China). The direct cAMP enzyme immunoassay kit (KGE002B) was purchased from R&D Systems (Minneapolis, MN, United States). The bicinchoninic acid protein assay kit (kw0014) was purchased from KWBIO (Beijing, China).
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5

Reagents for Cell Culture and Calcium Signaling

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Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum, penicillin, streptomycin, Hanks Balanced Salt Solution, bovine serum albumin (BSA), sodium pyruvate, and trypsin were purchased from Gibco (Carlsbad, CA, USA). Collagenase type IA, aluminum hydroxide gel, carbachol (CCH), IL-4, nifedipine, apamin, dimethyl sulfoxide, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, egtazic acid, and OVA were purchased from Sigma-Aldrich (St. Louis, MO, USA). NaCl, KCl, MgSO4, NaHCO3, KH2PO4, CaCl2, and glucose were purchased from Guangzhou Chemical Pharmaceutical Factory (Guangzhou, China). GSK5498A, BTP-2, and thapsigargin (TG) were purchased from MedChem Express (Monmouth Junction, NJ, USA). The IL-4 enzyme-linked immunosorbent assay kit was purchased from NeoBioscience (Shenzhen, China). Fluo8-AM was purchased from Abcam (Cambridge, UK).
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