RNA immunoprecipitation (RIP) experiments were performed using EZMagna RIP kit (Millipore, Billerica, MA, USA), according to the manufacturer's instructions. Briefly, HCT116 and LoVo cells were lysed using the RIP lysis buffer, and the lysates were incubated with magnetic beads containing specific antibodies (Ago, EZH2) or control IgG (Millipore). The antibody against EZH2 (Abcam) was used at a 1:200 dilution. Next, the beads were washed and incubated with Proteinase K for protein removal. Finally, RT-qPCR assay was used to measure the RNAs.
Antibody against ezh2
Manufactured by Abcam
Sourced in China
Antibody against EZH2, a catalytic subunit of the Polycomb Repressive Complex 2 (PRC2). This antibody is designed to specifically recognize and bind to the EZH2 protein.
Automatically generated - may contain errors
3 protocols using antibody against ezh2
1
RIP Assay for Protein-RNA Interactions
2
RIP Assay using EZ-Magna Kit
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According to former study, the EZ-Magna RIP kit (Millipore, China) was used for RIP assay [22] (link). Antibody against EZH2 (Abcam, Shanghai, China) and control IgG were used to capture immunoprecipitated RNAs. The captured RNAs were detected by RT-qPCR.
3
Chromatin Immunoprecipitation (ChIP) Protocol
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CHIP assay was carried out using an EZ-Magna ChIP TMA Chromatin Immunoprecipitation Kit (Millipore, Billerica, MA, USA) as described in former study [22] (link). Antibody against EZH2 (Abcam, Shanghai, China) and control IgG were used. The captured chromatin DNAs were detected by qPCR.
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