Anti grp78 goat polyclonal

The complete list of materials is in (Materials S1 in File S1). Primary Abs used in Western blot analyses were: anti-Grp94 rat monoclonal, anti-calnexin and anti-calreticulin rabbit polyclonal and anti-Grp78 goat polyclonal (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-Grp94 rabbit polyclonal (Enzo Life Sciences, Lausen, Switzerland). Secondary Abs were: anti-rabbit and anti-rat IgG biotin-conjugated (Vector Laboratories, Burlingame, CA, USA), anti-goat IgG alkaline phosphatase-conjugated (Santa Cruz Biotechnology) and extravidin alkaline phosphatase-conjugated (Sigma-Aldrich). Novex alkaline phosphatase chemiluminescent substrate was from Invitrogen. All other reagents were of the highest purity grade.

IHC was performed as described [23 (link)]. Briefly, antigen unmasking was carried out in citrate buffer pH 6.0 heated to > 85 °C for 10 min followed by washes in PBS. Primary antibody used was anti-Grp 78 goat polyclonal (Santa Cruz, SC-1051) diluted 1/300. Sections were then incubated with ABC reagent (Vector Laboratories, PK-6100) for 30 min and developed using the Vector VIP kit (Vector Laboratories, SK-4600). Negative control sections for IHC were performed with the appropriate serum minus the primary antibody. Tissue sections that were known to express the protein of interest were included as positive controls. Images were captured using the Carl Zeiss Axiovision microscope fitted with an Axiocam colour CCD camera and associated Axiovision software.