GM12878 cells, OCI-Ly7 cells, OCI-Ly10 cells and SUDHL-4 cells were obtained from American Type Culture Collection. GM12878 cells and SUDHL-4 cells were grown in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.); OCI-Ly10 cells were grown in Iscove's modified Dulbecco's medium (IMDM; Gibco; Thermo Fisher Scientific, Inc.); OCI-Ly7 cells were grown in IMDM with 0.3 g/ml glutamine. All media included 10% fetal bovine serum (HyClone; Cytiva), and all cells were cultured at 37˚C in a 5% CO2 incubator.
Gm12878 cells
Manufactured by Thermo Fisher Scientific
GM12878 cells are a human B-lymphoblastoid cell line derived from a female Caucasian individual. These cells are commonly used in genomic and epigenomic research.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hek293 cell, by Thermo Fisher Scientific (2 mentions)
Gm12878 cells, by Coriell Institute for Medical Research (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Hek293 cell, by American Type Culture Collection (2 mentions)
Hyclone, by Cytiva (1 mentions)
Sudhl 4 cells, by American Type Culture Collection (1 mentions)
3 protocols using gm12878 cells
1
Culturing B-cell Lymphoma Cell Lines
2
Single-Cell Isolation and Culture
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We used GM12878 cells (Coriell Institute) and HEK293 cells (American Type Culture Collection) for protocol optimization. Those cells were cultured at 37°C under 5% CO2 in a humidified incubator. We cultured GM12878 cells in RPMI 1640 medium (Gibco C11875500BT) with 10% fetal bovine serum (Gibco 10100147) and 1% penicillin–streptomycin (Gibco 15140122), then spun the cell suspension at 500g for 5 min, discarded the supernatant, and washed the cell pellet twice using PBS before resuspending it in PBS with 1% BSA. We cultured HEK293 cells in DMEM medium (Gibco 11965092) with 10% fetal bovine serum and 1% penicillin–streptomycin. The cells were then washed twice using PBS, detached by adding 1 mL 0.25% trypsin-EDTA (Gibco 25200056) to their culture dish, centrifuged at 500g for 5 min, and recovered in 1% PBS-BSA buffer. All cells underwent FACS that was gated for single cells, and each cell was subsequently sorted to a well in a 96-well plate.
3
Cell Preparation and FACS Sorting
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We used GM12878 cells (Coriell Institute) and HEK293 cells (American Type Culture Collection) for protocol optimization. Those cells were cultured at 37 ℃ under 5% CO2 in a humidified incubator. We cultured GM12878 cells in RPMI 1640 medium (Gibco, #C11875500BT) with 10% fetal bovine serum (Gibco, #10100147) and 1% penicillin-streptomycin (Gibco, #15140122), then spun the cell suspension at 500g for 5 min, discarded the supernatant, and washed the cell pellet twice using PBS before resuspending it in PBS with 1% BSA. We cultured HEK293 cells in DMEM medium (Gibco, #11965092) with 10% fetal bovine serum and 1% penicillinstreptomycin. The cells were then washed twice using PBS, detached by adding 1 mL 0.25% trypsin-EDTA (Gibco, #25200056) to their culture dish, centrifuged at 500g for 5 min, and recovered in 1% PBS-BSA buffer. All cells underwent FACS that was gated for single-cells and each cell was subsequently sorted to a well in a 96-well plate.
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