Anti cd11b biotin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-CD11b-biotin is a laboratory reagent that binds to the CD11b cell surface molecule, which is expressed on various immune cells, including monocytes, macrophages, and neutrophils. It is conjugated with biotin, a small molecule that can be detected using streptavidin-based detection systems. This product is intended for use in research applications involving the identification and characterization of cells expressing CD11b.

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Lab products found in correlation

Anti ter119 biotin, by Thermo Fisher Scientific (1 mentions) Anti cd11b apc, by Thermo Fisher Scientific (1 mentions) Anti cd11b pe, by Thermo Fisher Scientific (1 mentions) Anti cd11c pe, by Thermo Fisher Scientific (1 mentions) Anti f4 80 pe cy5, by Thermo Fisher Scientific (1 mentions) Anti cd45 pe cy7, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Biotin-conjugated anti-CD11b antibody CD11b-biotin Anti-CD11b CD11b

2 protocols using anti cd11b biotin

Magnetic T Cell and APC Enrichment

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T cells were magnetically enriched by negative selection from spleens of recipient mice following staining with anti-CD19-biotin (Fitch Monoclonal Facility), anti-Ter119-biotin (eBioscience), and anti-CD11b-biotin (eBioscience) and incubation with streptavidin magnetic beads (Pierce, Thermo Fisher Scientific). APCs were magnetically enriched by negative selection of splenocytes from CD45.1⁺ B6 mice following staining with anti-Thy1.2-biotin (eBioscience) and incubation with streptavidin magnetic beads (Pierce).

Miller M.L., McIntosh C.M., Williams J.B., Wang Y., Hollinger M.K., Isaad N.J., Moon J.J., Gajewski T.F., Chong A.S, & Alegre M.L. (2018). Distinct Graft-Specific TCR Avidity Profiles during Acute Rejection and Tolerance. Cell reports, 24(8), 2112-2126.

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Multicolor Flow Cytometry of Myeloid Cells

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Anti-CD11c-PE, anti-CD11b-Biotin (both eBioscience, San Diego, United States), and anti-F4/80 (clone CI:A3-1) and anti-Dectin-1 (clone 2A11), which were affinity-purified from hybridoma cell culture supernatants with protein G-Sepharose (Pharmacia, Uppsala, Sweden), were used for immunofluorescence. For FACS analysis anti-CD11c-Pacific Blue, anti-CD11b-APC, anti-CD11b-PE, anti-CD45-PE-Cy7, anti-F4/80-PE-Cy5 (all eBioscience, San Diego, United States), anti-Dectin-1-FITC (Serotec Oxford, United Kingdom) and unlabeled anti-CX3CR1 (Abcam, Cambridge, United Kingdom) were used. Live/Dead Fixable Near-IR stain fluorescence (Invitrogen, Landsmeer, Netherlands) was used to exclude dead cells.

Erkelens M.N., Goverse G., Konijn T., Molenaar R., Beijer M.R., Van den Bossche J., de Goede K.E., Verberk S.G., de Jonge W.J., den Haan J.M, & Mebius R.E. (2020). Intestinal Macrophages Balance Inflammatory Expression Profiles via Vitamin A and Dectin-1-Mediated Signaling. Frontiers in Immunology, 11, 551.

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